蒙药嘎日迪-15中多糖的研究

用热水提取蒙药嘎日迪-15中水溶性多糖，经SephadexC-25进行提纯精制得纯糖，采用硫酸-苯酚法测定了其水溶性多糖含量。方法的平均回收率为100.50%，RSD为0.82%。用GC测定了蒙药嘎日迪-15中水溶性多糖主要由木糖、甘露糖、半乳糖、葡萄糖组成，其摩尔比为1.03∶1.26∶0.77∶2.30 。用溴化钾压片法测得的红外谱图显示多糖的特征吸收峰为3 417.46，2 928.65，1 742.86，1 643.69，1 149.78, 1 078.19, 1 022.56，834.57 cm-1,其中1 078.19和1 022.56 cm-1为吡喃糖特征峰，834.57 cm-1是α-吡喃糖苷键的特征吸收峰。紫外谱图在280 nm处有明显的糖吸收峰，说明有CO键存在。

Determination of Constituents of Polysaccharide and the Content of Saccharide in Mongolian Medicine Garidi-15

The present paper introuduces the method, with which the water-soluble polysaccharide was extracted with hot water from Garidi-15, the products were further purified with column chromatography on SephadexC-25, and the contents of water soluble polysaccharide were determined by phenyl hydrate-sulfuric acid method. The average recovery was 100.50%, and RSD was 0.82%. The components of water soluble polysaccharide were identified by gas chromatography as xylose, mannose, galactose and glucose in the molar ratio of 1.03 : 1.26 : 0.77 : 2.30. The water soluble polysaccharide was identified by IR spectrum and UV(200-400 nm) scanning spectrum. The IR spectrum indicates that there are characteristic absorption peaks of polysaccharides at 3 417.46, 2 928.65, 1 742.86, 1 643.69, 1 149.78, 1 078.19, 1 022.56 and 834.57 cm(-1), of which there are absorption peaks of pyranose at 1 078.19 and 1 022.56 cm(-1), and contain one characteristic absorption peak of alpha-pyranose indican band at 834.57 cm(-1). The UV scanning spectrum shows the obvious polysaccharide absorption peak at 280 nm, and indicates the existence of C=O bond.