首页 | 本学科首页   官方微博 | 高级检索  
     检索      

分子印迹技术特异性识别头孢类分子的光谱研究
作者姓名:Jiang YX  Pang DL  He Q  Lai CY  Zheng HL  Li F
作者单位:1. 四川大学化学学院,绿色化学与技术教育部重点实验室,四川成都610064
2. 重庆大学三峡库区生态环境教育部重点实验室,重庆,400045
基金项目:国家水体污染控制与治理科技重大专项项目
摘    要:以头孢氨苄(CFL)为特异性分析识别对象,采用本体聚合和悬浮聚合两种聚合方法,制备了头孢氨苄分子印迹聚合物.紫外光谱研究证实模板分子CFL与内烯酰胺之间形成氢键型配合物.用红外光谱法分析了CFL分子印迹聚合物的聚合程度及聚合物表面上存在的功能基团.紫外光谱分析表明,用AM-EGD-MA体系和本体聚合法制备的印迹聚合物对...

关 键 词:分子印迹聚合物  头孢氨苄  光谱表征  固相萃取

Spectroscopy study on the selectively distinguishing cefalexin with the molecular imprinted polymer
Jiang YX,Pang DL,He Q,Lai CY,Zheng HL,Li F.Spectroscopy study on the selectively distinguishing cefalexin with the molecular imprinted polymer[J].Spectroscopy and Spectral Analysis,2011,31(7):1852-1856.
Authors:Jiang Yu-xiang  Pang Dao-lin  He Qiang  Lai Cheng-yue  Zheng Huai-li  Li Fang
Institution:College of Chemistry, Key Laboratory of Green Chemistry and Technology, Ministry of Education, Sichuan University, Chengdu 610064, China. hanbing820217@163.com
Abstract:Molecular imprinted polymers of cefalexin (CFL) were prepared by non-covalent molecular imprinting technique in the present paper. Using CFL as template molecule, acrylamide (AM) or methacrylic acid (MAA) as functional monomer, ethylene dimethacrylate (EGDMA) as cross-linker, AIBN as initiator and methanol as porogen agent, different molecularly imprinted polymers (MIPs) of CFL were synthesized by bulk or suspension polymerization as synthetic method. The intermolecular action between AM and CFL was investigated by UV and IR spectrophotometric analysis, and the results indicated that polymerizing functional monomer AM could bond effectively with template molecule CFL. By using UV spectrophotometric analysis method, it was found that the MIP prepared with AM-EGDMA by bulk polymerization showed the highest binding capacity for CFL. Test of selective adsorption made clear that the MIP represented more excellent identification property to CFL than to cefadroxol and ampicillin. The MIPs were used as solid-phase extraction sorbent for extraction and enrichment of CFL in actual samples. And the recoveries for CFL extraction were found to be 99.3%-99.7% (n=3), demonstrating the feasibility of the prepared MIPs for CFL extraction.
Keywords:
本文献已被 万方数据 PubMed 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号