三维荧光光谱结合二阶校正算法测定碳酸饮料中胭脂红的含量

利用三维荧光光谱结合交替归一加权残差算法(ANWE)，对碳酸饮料中胭脂红含量的直接测定。首先通过使用英国爱丁堡公司生产的FLS920P荧光光谱仪测量所配制的胭脂红和日落黄混合溶液样品的三维荧光光谱，利用ANWE算法来进行解析，得到校正集中浓度与真实浓度的相关系数为0.9917，平均回收率为100.92%±2.71%，结果表明，ANWE算法可靠性比较好;然后把市售碳酸饮料稀释8，9，12，13倍，分别测量它们的三维荧光光谱，结合ANWE算法进行解析，计算得到校正集中浓度和实际浓度的相关系数分别为0.993 0，0.993 0，0.993 2，0.993 2，以及饮料中胭脂红含量分别为38.88，37.71，37.68和39.65 μg·mL-1，平均浓度为(38.48±0.96) μg·mL-1;最后，为了验证所得饮料中胭脂红浓度的准确性，使用标准添加法，解析得到，校正样品中胭脂红的校正浓度和真实浓度相关系数为0.993 5，且平均回收率为102.99%±2.15%。检测结果可为饮料中食品色素的快速定量提供一种新的思路。

Determination of Carmine in Carbonated Beverages Using 3-D Fluorescence Spectra Coupled with Second-Order Calibration Algo ri thm

Three-dimensional fluorescence spectra combined with second-order calibration algorithm based on alternate a weighted residual (ANWE) was applied to the direct concentration determination of carmine in carbonated beverages. Firstly, 3-D fluorescence spectra of carmine and sunset yellow mixed solutions with different concentrations were obtained by employing spectrometer, and analyzed by using ANWE, the correlation coefficient between calibration concentration and the actual concentration was 0.991 7, and the average recovery was 100.92%±2.71%. The results show that the ANWE algorithm is reliable. Then, the commercial carbonated soft drinks in 8, 9, 12 and 13 times diluted concentration were detected by using ANWE algorithm, the correlation coefficient between relative concentration and the actual concentration were 0.993 0, 0.993 0, 0.993 2 and 0.793 2, respectively, and the contents of carmine in beverage were 38.88, 37.71, 37.68 and 39.65 μg·mL-1, respectively. The average concentration was (38.48±0.96) μg·mL-1. Finally, the standard addition method was applied to estimate the prediction accuracy between calibration concentration and the actual concentration was 0.993 5, and the average recovery was 102.99%±2.15%. The results can provide a new idea for the rapid content determination of food pigments in commercial beverages.