面向生物过程的阿达玛变换近红外光谱仪

对生物过程中营养物质的消耗进行监测，有利于控制微生物的生长环境，使其始终处于最佳生长条件，从而使目标产物的产量最大化。针对酵母培养液中甘油、甲醇和葡萄糖含量的监测需求，研制了一种基于阿达玛变换(hadamard transform，HT)技术的近红外光谱仪。该光谱仪使用自主改进设计的近红外原位探头进行光谱信号的收集，采用数字微镜(digital micro-mirror devices，DMD)进行阿达玛模板的编码调制，配合自主开发的光谱采集软件和光谱分析处理软件，实现了培养液中营养物含量的实时监测。给出了光谱仪的光路设计、近红外原位探头改进设计、硬件电路和软件模块的设计。通过实验测得该光谱仪的杂散光为0.875%，波动率为±4.28%。利用该光谱仪对一系列标准浓度的甘油水溶液进行光谱采集和数学建模，实验结果表明该仪器测量准确，能够满足生物过程监测的要求。

Hadamard Transform NIR Spectrometer for the Biological Processes

Monitoring the consumption of nutrients of biological process helps control the growth environment of the microbes. It ensures the microbes are always in the best growing conditions, so as to maximize the yield of the target product. To monitor the content of glycerol, methanol and glucose in yeast culture medium, a new NIR spectrometer was developed which is based on Hadamard Transform(HT)technique. It uses the Near IR probe, which was designed all by ourselves, to collect the spectral signals, and uses the Digital Micro-mirror Devices(DMD)to complete the coding and modulation of Hadamard template. With self-developed software, aiming at spectral acquisition and spectral processing, the spectrometer has realized the real-time monitoring. The designs of optical path, Near IR probe, hardware circuit and software modules are given. Through lots of experiments, it turns out that the value of stray light is 0.875% and the volatility is ±4.28% in this spectrometer. The spectrometer shows high precision in a series of tests which means it totally meets the requirements of monitoring the biological processes.