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| 臭氧对胎牛血清氧化损伤的表面增强拉曼光谱 | |
| 引用本文： | 邹祖全,刘燕楠,吴瑛,李奇楠,陈梅宗,孙海英,李任植.臭氧对胎牛血清氧化损伤的表面增强拉曼光谱[J].光谱学与光谱分析,2007,27(6):1140-1142. |
| 作者姓名： | 邹祖全 刘燕楠 吴瑛 李奇楠 陈梅宗 孙海英 李任植 |
| 作者单位： | 1. 南开大学物理科学学院生物物理系,天津,300071;南开大学物理科学学院光子学中心,天津,300071 2. 南开大学物理科学学院光子学中心,天津,300071;天津市南开大学弱光非线性光子学材料先进技术及制备教育部重点实验室,天津,300457 3. 国家纳米技术与工程研究院,天津,300457 4. 南开大学物理科学学院生物物理系,天津,300071;天津市南开大学弱光非线性光子学材料先进技术及制备教育部重点实验室,天津,300457 5. 南开大学物理科学学院生物物理系,天津,300071;天津市南开大学弱光非线性光子学材料先进技术及制备教育部重点实验室,天津,300457;南开大学生物活性材料教育部重点实验室,天津,300071 |
| 摘 要： | 对实验室细胞培养用的国产胎牛血清在臭氧作用前后进行了表面增强拉曼光谱测试,范围200～1 800 cm-1.发现臭氧作用1 h后拉曼谱线强度大大减弱,作用3 h后谱线强度进一步减弱,但减弱的程度明显减慢,说明臭氧氧化作用具有快速的特点.臭氧作用下蛋白质主链的有序构象(α-螺旋,β折叠)明显减少,对β折叠的损伤更为严重,β回折几乎消失.芳香族氨基酸和半胱氨酸侧链在臭氧作用下氧化损伤非常明显.这些特征谱线的变化,反映了臭氧的氧化作用使蛋白质变性,构象变化以及降解. |
| 关 键 词： | 表面增强拉曼光谱 胎牛血清 臭氧 |
| 文章编号： | 1000-0593（2007）06-1140-03 |
| 修稿时间： | 2005-12-182006-03-10 |
Surface-Enhanced Raman Spectra of Oxidation Damnificaion of Fetal Bovine Serum by Ozne |
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| ZOU Zu-quan,LIU Yan-nan,WU Ying,LI Qi-nan,CHEN Mei-zong,SUN Hai-ying,Lee Imshik.Surface-Enhanced Raman Spectra of Oxidation Damnificaion of Fetal Bovine Serum by Ozne[J].Spectroscopy and Spectral Analysis,2007,27(6):1140-1142. | |
| Authors: | ZOU Zu-quan LIU Yan-nan WU Ying LI Qi-nan CHEN Mei-zong SUN Hai-ying Lee Imshik |
| Institution: | 1. Department of Biophysics, School of Physics, Nankai University, Tianjin 300071, China; 2. Photonics Center of School of Physics, Nankai University, Tianjin 300071, China ;3. The Key Laboratory of Advanced Technique and Fabrication for Weak-Light Nonlinear Photonics Materials, Ministry of Education, Nankai University, Tianjin 300457, China; 4. The Key Laboratory of Bioactive Material, Ministry of Education, Nankai University, Tianjin 300071, China; 5. China National Academy of Nanotechnology and Engineering, Tianjin 300457, China |
| Abstract: | Fetal bovine serum was treated by ozone for 1 hour and 3 hours before getting its surface-enhanced Raman spectra from 200 to 1 800 cm(-1). Treated with ozone for 1 hour, it shows a significant decrease in band intensity. Treated with ozone for 3 hours, the band intensity has a further decrease but not so obviously, which means that oxidation of ozone is short lived. Treated with ozone, the orderly conformations of main chains in protein such as alpha-helix, beta-sheet and beta-corner are damaged seriously. Aromatic side chains and C-S of Cys and Met also are damnified greatly. All this means that strong oxidation of ozone results in denaturation, conformational changes and even degradation in protein. |
| Keywords: | Surface-enhanced Raman spectra Fetal bovine serum Ozone |
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