| 肺癌和癌旁组织中55个元素的原子光谱分析 |
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| 引用本文： | 熊依杰,欧阳荔,刘雅琼,解清,刘虎生,王京宇.肺癌和癌旁组织中55个元素的原子光谱分析[J].光谱学与光谱分析,2006,26(11):2120-2124. |
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| 作者姓名： | 熊依杰 欧阳荔 刘雅琼 解清 刘虎生 王京宇 |
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| 作者单位： | 1. 北京大学公共卫生学院,北京,100083
2. 北京大学公共卫生学院,北京,100083;北京大学医药分析中心生命元素组学实验室,北京,100083 |
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| 基金项目： | 教育部留学回国人员科研启动基金 |
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| 摘 要： | 建立了利用原子光谱技术测定人肺组织中55种元素的方法.样品采用冷消化过夜,温控电热板消解.其中49种元素利用电感耦合等离子体质谱(ICP-MS)进行测定;As和Se由原子荧光分光光度计(AFS)测定,K,Na,Ca和Mg由原子吸收分光光度计(AAS)测定.24个元素的测定值与牛肝和人发两种标准物质中的参考值基本吻合.除了Cr,Er和U三元素回收率略为偏高外,其余30种元素的加标回收率均在90%～110%之间.绝大多数元素的精密度在1.7%～10%之间.统计学分析结果表明,共有20种元素的含量在所测17对癌组织和癌旁组织之间具有显著性差异.此方法简便、快速、准确.
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| 关 键 词： | 肺癌 原子光谱分析 多元素 |
| 文章编号： | 1000-0593（2006）11-2120-05 |
| 收稿时间： | 2005-12-26 |
| 修稿时间： | 2006-04-18 |
Determination of Fifty-Five Elements in Lung Carcinomatous Tissues and Their Pericarcinomatous Tissues by Atomic Spectrometry |
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| XIONG Yi-jie,OUYANG Li,LIU Ya-qiong,XIE Qing,LIU Hu-sheng,WANG Jing-yu.Determination of Fifty-Five Elements in Lung Carcinomatous Tissues and Their Pericarcinomatous Tissues by Atomic Spectrometry[J].Spectroscopy and Spectral Analysis,2006,26(11):2120-2124. |
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| Authors: | XIONG Yi-jie OUYANG Li LIU Ya-qiong XIE Qing LIU Hu-sheng WANG Jing-yu |
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| Institution: | 1. School of Public Health, Peking University, Beijing 100083, China; 2. Laboratory of Biometallomies, Center of Medical and Health Analysis, Peking University, Beijing 100083 |
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| Abstract: | A method for determining 55 elements in human lung tissue was developed. Mixed acid (HNO3:HCl04) was added into samples, which were digested at room temperature over night, then heated at 180 degrees C. Arsenic and selenium in lung tissue were determined by hydride generation atomic fluorescence spectrometry (HG-AFS), potassium, sodium, calcium and magnesium were determined by atomic absorption spectrometer (AAS), while the rest of forty-eight elements were determined by inductively coupled plasma mass spectrometry (ICP-MS), respectively. Reference materials of GBW(E)080193 bovine hepar and GBWO9101 human hair were analyzed by the described method. The measured element values in two reference materials accorded with their reference values. The recovery rates for most of the studied elements were 90%-110%. The precisions of the method were 1.7%-10.0%. The concentrations of seventeen elements in the carcinomatous tissues were remarkably different from those in the pericarcinomatous tissues. The method is rapid, simple and accurate. |
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| Keywords: | Lung cancers Atomic spectrometry Elements |
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