| 植物中硝态氮、氨态氮、总氮测定方法的比较研究 |
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| 引用本文： | 吕伟仙,葛滢,吴建之,常杰.植物中硝态氮、氨态氮、总氮测定方法的比较研究[J].光谱学与光谱分析,2004,24(2):204-206. |
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| 作者姓名： | 吕伟仙 葛滢 吴建之 常杰 |
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| 作者单位： | 1. 浙江大学生命科学学院,浙江,杭州,310029
2. 浙江大学分析测试中心,浙江,杭州,310028 |
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| 基金项目： | 国家重点基础研究规划项目 (G2 0 0 0 0 4680 5 )，国家自然科学基金项目 ( 3 9970 0 5 8)资助 |
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| 摘 要： | 植物样品经硫酸 过氧化氢凯氏法消煮后 ,分别用直接吸光光度法、靛酚蓝比色法、过硫酸钾氧化吸光光度法获得硝态氮 (包括亚硝态氮 )、氨态氮、总氮的含量。通过样品测试数据的比较 ,证明分别测得的氨态氮、硝态氮含量之和与所测总氮结果一致。本文为植物生态学和植物学中氮元素各种形态含量的分析提供了选择测定方法的依据
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| 关 键 词： | 植物 凯氏法 硝态氮 氨态氮 总氮 |
| 文章编号： | 1000-0593(2004)02-0204-03 |
| 修稿时间： | 2002年11月5日 |
Study on the Method for the Determination of Nitric Nitrogen,Ammoniacal Nitrogen and Total Nitrogen in Plant |
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| Wei-xian Lü,Ying Ge,Jian-zhi Wu,Jie Chang.Study on the Method for the Determination of Nitric Nitrogen,Ammoniacal Nitrogen and Total Nitrogen in Plant[J].Spectroscopy and Spectral Analysis,2004,24(2):204-206. |
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| Authors: | Wei-xian Lü Ying Ge Jian-zhi Wu Jie Chang |
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| Institution: | College of Life Science, Zhejiang University, Hangzhou 310029, China. |
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| Abstract: | This paper reports the determination of three nitrogen states in plants: nitric nitrogen, ammoniacal nitrogen and total nitrogen. The plant sample was digested by the Kjeldathl method with H 2SO 4 H 2O 2. The solution of the sample was determined by three methods: absorption photometric method, indigotic colorimetry, and UV absorption photometric method after K 2S 2O 8 oxidation. By comparing the results, the relationship among the three N states is that the total N= NH 4 NO - 3. Suitable methods were found for the determination of the elements, thus providing a scientific basis for the selection of suitable methods for chemical element analysis in plants. |
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| Keywords: | Plant Kjeldathl method Nitric nitrogen Ammoniacal nitrogen Total nitrogen |
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