| 达旦黄-曲通X-100体系共振光散射法测定蛋白质 |
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| 引用本文： | 冯素玲,王瑾,樊静.达旦黄-曲通X-100体系共振光散射法测定蛋白质[J].光谱学与光谱分析,2005,25(6):927-929. |
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| 作者姓名： | 冯素玲 王瑾 樊静 |
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| 作者单位： | 河南师范大学化学与环境科学学院,河南省高等学校环境科学与工程重点学科开放实验室,河南,新乡,453002 |
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| 基金项目： | 河南省自然科学基金(0211020200)资助项目 |
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| 摘 要： | 基于在TritonX 10 0存在下,蛋白质与达旦黄作用使得体系的共振光散射增强,在λ=4 92nm处光散射强度最大,增强作用的强弱与蛋白质的含量成正比,据此建立了共振光散射测定蛋白质的新方法。此方法对牛血清白蛋白的检出限达到17 7ng·mL- 1 ,线性范围为0 0 3～0 9μg·mL- 1 ,用于合成样与人血清样品的分析,取得了令人满意的结果。同时也研究了人血清白蛋白、鸡蛋白蛋白、溶菌酶、胰蛋白酶与达旦黄的作用。
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| 关 键 词： | 共振光散射 蛋白质 达旦黄 曲通X100 |
| 文章编号： | 1000-0593(2005)06-0927-03 |
| 修稿时间： | 2003年12月6日 |
Microdetermination of Proteins with Titan Yellow by the Resonance Light Scattering Method |
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| FENG Su-ling,WANG Jin,FAN Jing.Microdetermination of Proteins with Titan Yellow by the Resonance Light Scattering Method[J].Spectroscopy and Spectral Analysis,2005,25(6):927-929. |
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| Authors: | FENG Su-ling WANG Jin FAN Jing |
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| Institution: | College of Chemistry and Environmental Science, Key Laboratory of Environmental Science and Engineering of Henan Education Department, Henan Normal University, Xinxiang 453002, China. |
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| Abstract: | A resonance light scattering method for the determination of trace proteins was developed. In the presence of Triton X-100, proteins reacted with Titan yellow to form a combination product, resulting a significant enhancement of resonance light scattering (RLS). The deltaI(RLS) was directly proportional to the concentration of protein in the range of 0.03-0.9 microg x mL(-1), with the detection limit 17.7 ng x mL(-1) for BSA. This method was applied to the determination of the proteins in synthetic and human serum samples, and compared to the CBB method, with satisfactory results. |
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| Keywords: | Resonance light scattering Proteins Titan yellow Triton X-100 |
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