麻痹性贝毒产毒藻株的三维荧光光谱识别技术研究

利用三维荧光光谱技术，研究了以微小亚历山大藻 （台湾株）（AMSY）、塔玛亚历山大藻（大亚湾株）（ATDY）、链状裸甲藻（防城港株）（GCFC）、塔玛亚历山大藻（香港株）（ATHK）、链状亚历山大藻（南海株）（ACSY）5株4种产麻痹性贝毒(PSP)微藻以及21株不产PSP藻在不同温度条件下培养微藻的各生长期产毒能力与三维荧光的关系。研究结果表明，在不同温度条件下，产毒藻类单位藻细胞产毒量会发生显著变化，低温可促进麻痹性贝毒的产生。通过Db7小波分解，选取Ca3尺度分量的联合荧光谱作为特征谱并利用Fisher判别发现，产PSP藻与不产PSP藻荧光差异主要集中在λ_ex为400~425和450~545nm；λ_em为715~750 nm的波段，利用判别函数建立判别式，实现了对产麻痹性贝类毒素藻类的识别测定。对产PSP毒素藻类的判别率达到93.7%，不产PSP毒素藻类判别率达到93.3% ，综合判别率为93.5%。该研究可实现对水体中活体产PSP微藻的快速识别，为进一步开发产毒微藻识别仪提供理论基础。

Identification of Paralytic Shellfish Algae by Three-Dimensional Fluorescence Spectral

Using three-dimensional fluorescence spectroscopy technology,the relationship between three-dimensional fluorescence and toxicity of 5 strains of 4 microalgae species producing paralytic shellfish poisoning(PSP),which were Alexandrium minutum(Taiwan strain,AMSY),Alexandrium tamarense(Daya Bay strain,ATDY),Gymnodinium catenatum(Fangcheng Lane strain,GCFC),Alexandrium tamarense(Hong Kong strain,ATHK),Alexandrium catenella(South China Sea strain,ACSY),and 21 non-producing PSP microalgae species under different temperature conditions culture was studied.The results show that the toxin content of toxic algae per cell would change significantly under different temperature culture conditions and low temperature could promote the PSP production of toxic microalgae.By Db7 wavelet decomposition,the combined fluorescence spectrum of Ca3 scale components was selected as the characteristic spectrum and was used to establish the Fisher discrimination models,The combined fluorescence spectrum differences between toxic and non-toxic microalgae focused mainly on λ_ex 400~425,450~545 nm and λ_em 715~750 nm bands.Using the Fisher discrimination models to dentification the toxic and non-toxic microalgae,the identification rate reached 93.7%and 93.3%,respectively,and the comprehensive discrimination rate was up to 93.5%.The method could be used to rapidly identify toxic microalgae in sea water and provide the theoretical basis for further developing toxic microalgae identification instruments.