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紫外辐射对小牛胸腺DNA水溶液影响的拉曼光谱研究
引用本文:周殿凤,柯惟中.紫外辐射对小牛胸腺DNA水溶液影响的拉曼光谱研究[J].光谱学与光谱分析,2004,24(11):1370-1372.
作者姓名:周殿凤  柯惟中
作者单位:南京师范大学,江苏省光电技术重点实验室,江苏,南京,210097
基金项目:江苏省自然科学基金,江苏省高校重点实验室科研基金资助项目
摘    要:报道了小牛胸腺DNA水溶液经9,20,40min紫外辐射的拉曼光谱图,紫外线的辐射照度为1868W·m-2。实验结果表明波长为2537nm的紫外光在起主要作用的紫外辐射对DNA的损伤是严重的,短短10min的紫外辐射就使1094cm-1这个强峰分裂成几个小峰,说明DNA的构象受到破坏,DNA的构型发生变化,部分单双键发生了断裂,出现了各种各样由于DNA键断裂产生的多核苷酸;4种碱基均受到不同程度的影响,碱基间的氢键造成断裂,其中嘧啶、嘌呤碱基受到的损伤较为严重;紫外辐射对脱氧核糖也产生了破坏。另外,该实验也表明,在水溶液中,DNA以B型结构为主,局部的A型结构仍然存在。

关 键 词:严重  DNA  损伤  多核苷酸  脱氧核糖  局部  影响  拉曼光谱  水溶液  嘧啶
文章编号:1000-0593(2004)11-1370-03
修稿时间:2003年6月18日

Raman Spectroscopic Study on the Influence of Ultraviolet Radiation on Calf Thymus DNA in Aqueous Solution
ZHOU Dian feng,KE Wei zhong Jiangsu Provincial Key Lab for Optoelectric Technology,Nanjing Normal University,Nanjing ,China.Raman Spectroscopic Study on the Influence of Ultraviolet Radiation on Calf Thymus DNA in Aqueous Solution[J].Spectroscopy and Spectral Analysis,2004,24(11):1370-1372.
Authors:ZHOU Dian feng  KE Wei zhong Jiangsu Provincial Key Lab for Optoelectric Technology  Nanjing Normal University  Nanjing  China
Institution:Jiangsu Provincial Key Lab for Optoelectric Technology, Nanjing Normal University, Nanjing 210097, China.
Abstract:The Raman spectra of calf thymus DNA in aqueous solution and at 9, 20 and 40 min after ultraviolet radiation were reported. The experimental results proved that though the ultraviolet radiation time was not long, it had a serious influence on the DNA. Only through 9 min of ultraviolet radiation, the peak of 1 094 cm -1 changed into a few weaker peaks, and it was proved that the molecular conformation was changed and the hydrogen bonds were damaged. Some kinds of nucleotides came into being because of the damage to the DNA molecular. Ultraviolet radiation also had influence on adenine, guanine, cytosine and thymine. Among them the purine and pyrimidine were badly damaged. At the same time, our experiment proved that DNA in aqueous solution possesses both A type structure and B type structure.
Keywords:Ultraviolet radiation  Calf thymus DNA  Raman spectra
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