应用SERS滤纸基底检测饮料中违禁色素的研究

利用液/液界面自组装技术制备得到灵敏度高、均匀性好、价格低廉的表面增强拉曼光谱(SERS)滤纸基底,并使用该基底检测了饮料中可能掺杂的罗丹明B、日落黄和柯衣定等三种色素。首先分析了罗丹明B、日落黄和柯衣定的分子结构并对其进行了拉曼特征峰峰位归属;然后检测了罗丹明B、日落黄和柯衣定不同浓度水溶液的SERS光谱;最后在无任何预处理条件下,检测了饮料中的罗丹明B、日落黄和柯衣定含量。在一定浓度范围内,饮料中三种色素的浓度与其SERS特征峰强度分别满足一定的函数关系,其中罗丹明B和日落黄的浓度与拉曼特征峰强度之间呈非线性关系,而柯衣定的浓度与拉曼特征峰强度之间呈线性关系。评估了本方法检测饮料中的罗丹明B、日落黄、柯衣定的信号重复性及检测回收率,结果表明SERS方法可用来对饮料中罗丹明B、日落黄、柯衣定的浓度进行半定量分析。为饮料中添加色素的现场实时检测提供了一种简便快速高效的检测方式,可用于饮料的质量控制及市场监控。

Study on Detection of Prohibited Pigments in Drinks Using Paper-Based SERS Substrates

In this paper ,a novel paper‐based Surface enhanced Raman spectroscopy (SERS) substrate with high sensitivity , good uniformity and popular price is developed via liquid /liquid interface self‐assembly technique .Three pigment ,rhodamine B , sunset yellow and chrysoidine were detected through paper‐based SERS substrates using a portable Raman spectrometer .The structures of the three pigments were investigated and vibrational modes of characteristic peaks of three pigments were assigned . SERS spectra of rhodamine B ,sunset yellow and chrysoidine in aqueous solution with different concentrations were detected re ‐spectively .Rhodamine B ,sunset yellow and chrysoidine in drinks were also detected in drinks without any pretreatment .Within a certain range of concentrations ,it meets certain function .For rhodamine B and sunset yellow ,the relationship between concen‐tration and Raman peak intensity is on an index curve ,while for chrysoidine ,the relationship is linear .In addition ,high recover‐ies are achieved for detecting rhodamine B ,sunset yellow and chrysoidine in drinks ,which indicated our method is suited for semi‐quantitative analysis for the concentration of rhodamine B ,sunset yellow and chrysoidine in drinks .Surface‐enhanced Ra‐man spectroscopy provides an easy approach to fast and efficient detection for multiple pigments in drinks and can be used for quality control and market monitoring of drinks .