内分泌干扰物三苯基锡的激光拉曼光谱分析

三苯基锡(TPhT)是目前已知的内分泌干扰物中唯一的两种金属化合物之一，被广泛应用于工业、农业和交通领域，其大量使用会对土壤、海洋和内陆淡水环境造成不同程度的影响。本实验采用激光共聚焦拉曼光谱采集固体TPhT的拉曼光谱信号，尝试将该方法用于TPhT检测，探索该方法的可行性，并进行检测参数的优化选择。将拉曼光谱分析检测方法与TPhT的物性研究相结合，根据TPhT分子中不同官能团振动模式的不同，将拉曼谱图分为高、中、低3个波数区(1 500～3 200，900～1 500和100～900 cm-1)进行拉曼谱峰的归属与分析，得到了TPhT的特征振动模式和拉曼特征峰，并建立一套TPhT的标准拉曼图谱库，光谱范围在100～3 200 cm-1之间。结果表明，当激光功率选择为衰减到原激光功率(500 mW)的0.5%、曝光10 s、累积2次时，得到的拉曼谱图信噪比高且检测时间短。在212，332，657，997和1 577 cm-1处出现的信号强度较高的拉曼峰，可作为固体TPhT拉曼检测的特征峰，657和997 cm-1处拉曼特征峰的同时出现即可认为复杂的环境样品中存在TPhT。实验结果给出了辨别TPhT存在的标志，这些结果将为拉曼光谱用于实际环境样品中TPhT的残留检测提供理论依据和数据基础。

Analysis of Endocrine Disruptor: Triphenyltin Chloride with Raman Spectroscopy

Triphenyltin Chloride (TPhT)is one of the only two kinds of metal compounds known in the endocrine disruptors. TPhT is widely used in industry,agriculture and transportation fields,which can cause great impact on soil,marine and inland freshwater environment.This experiment collects the Raman signal of TPhT standard powders by applying laser confocal Raman spectroscopy to explore the feasibility of the method and to acquire optimized parameters.In this paper,we combined the appli-cation of laser confocal Raman spectroscopy with TPhT physical property.Due to different functional groups of TPhT molecules shows different vibration modes,the Raman spectra was divided into three wavenumber areas(1 500 ~ 3 200,900 ~ 1 500 and 100~900 cm-1 )to attribute and analyze their assignments of the Raman peaks,obtaining the characteristics of TPhT vibration modes and the corresponding characteristic peaks.Finally,a standard Raman spectra library was established with the spectral range between 100~3 200 cm-1 .The results showed that the Raman spectra with high signal-to-noise ratio can be obtained in a short time when the laser power options decayed to 0.5% of the original power (500 mW)after 10s exposure and 2 times inte-gration.Strong Raman signals observed in the Raman spectroscopy at 212,332,657,997 and 1 577 cm-1 could be used as the characteristic peaks of TPhT in Raman detection.Otherwise,the co-occurrence of the Raman peaks at 657 and 997 cm-1 can be considered as the presence of TPhT in complex environmental samples.Experimental results are given to identify the presence of TPhT.The results would be used to identify the presence of TPhT residual in real environmental samples,which provide a theo-retical basis and data base on Raman spectroscopy.