拉曼光谱在天然纤维素结构研究中的应用进展

纤维素是木质纤维生物质细胞壁的骨架物质，也是生物燃料制备过程中重要的前驱体。作为重要的天然有机高分子，纤维素分子结构的研究备受关注。拉曼光谱仪因其较高的分辨率及无损检测的特点可在多尺度研究天然纤维素复杂分子链及聚集态结构。本文在比较了色散型拉曼光谱仪和傅里叶变换拉曼光谱仪的构造及相关参数的基础上，详细综述了拉曼光谱技术在植物细胞壁纤维素微区分布、天然纤维素酶解发酵、分子链空间取向、分子形变、结晶度与多晶态转变等方面的研究进展。并对拉曼光谱技术在天然纤维素分子结构研究中存在的问题进行了总结，提出了可能的解决方案，以促进拉曼光谱技术在天然有机高分子研究领域的应用。

Study on the Application of Raman Spectroscopy to the Research on Natural Cellulose Structure

As the skeleton substances of lignocellulosic biomass cell wall and the precursor of biofuels production ,the research on cellulose structure ,an important natural biomarcromolecules ,attracts great attention .Considering its in situ features and higher resolution ,Raman spectroscopy has been used to investigate the structure of cellulose molecular chain and cellulose aggre ‐gation structure at multi‐scale .In this paper ,the configurations and corresponding parameters of two types of Raman spectros ‐copy (Dispersive Raman and FT‐Raman) were compared .Subsequently ,the utilization of Raman spectroscopy in cellulose micro‐distribution ,cellulose enzyme hydrolysis ,cellulose chain orientation and deformation ,cellulose crystallinity and polymorphic transformation was discussed in detail .Given the existing deficiencies of the Raman spectroscopy when used to investigate the natural cellulose ,some suggestions were proposed in order to promote the application of Raman spectroscopy to the research of natural macromolecular .