| 固定化木瓜蛋白酶活性的X-射线微区分析 |
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| 引用本文： | 丁良,姚子华,李彤,仇满德.固定化木瓜蛋白酶活性的X-射线微区分析[J].光谱学与光谱分析,2003,23(2):400-402. |
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| 作者姓名： | 丁良 姚子华 李彤 仇满德 |
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| 作者单位： | 1. 河北大学理化分析中心,河北,保定,071002;河北省职工医学院,河北,保定,071000
2. 河北大学理化分析中心,河北,保定,071002 |
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| 摘 要： | 利用X-射线微区分析,对共价法得到的固定化木瓜蛋白酶的活性进行了分析;Na-苯甲酰-L-精氨酰胺盐酸盐作为底物,FeCl_3作为捕捉剂,底物经木瓜蛋白酶催化分解产生L-精氨酸及氨,后者和捕捉剂反应产生沉淀,可以确定固定化木瓜蛋白酶的催化活性部位。结果表明:以大孔结构吸附树脂为固定化酶载体,酶活较高,木瓜蛋白酶分布较均匀。并得到了固定化木瓜蛋白酶的活性定位的最佳条件。并对不同结构载体固定化木瓜蛋白酶的活性进行了研究。
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| 关 键 词： | X-射线微区分析 固定化木瓜蛋白酶 大孔树脂 酶活 |
| 文章编号： | 1000-0593(2003)02-0400-03 |
| 修稿时间： | 2002年7月8日 |
X-Ray Microanalysis of the Activity of Immobilized Papain |
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| DING Liang,YAO Zi-hua,LI Tong and QIU Man-de
. Research Center of Physical and Chemical Analysis,Hebei University,Baoding ,China
. Hebei Medical Continued Education College,Baoding ,China.X-Ray Microanalysis of the Activity of Immobilized Papain[J].Spectroscopy and Spectral Analysis,2003,23(2):400-402. |
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| Authors: | DING Liang YAO Zi-hua LI Tong and QIU Man-de
Research Center of Physical and Chemical Analysis Hebei University Baoding China
Hebei Medical Continued Education College Baoding China |
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| Institution: | Research Center of Physical and Chemical Analysis, Hebei University, Hebei Medical Continued Education College, Baoding 071002, China. |
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| Abstract: | The localization of activity of immobilzed papain by covalent-binding was studied by X-ray microanalysis. Na-benzoyl-L-arginineamide hydrochloride and FeCl3 served as substrate and capturer respectively. Substrate was catalysed by immobilized papain to produce L-arginine and NH3. NH3 was captured by FeCl3 to form precipitate Fe(OH)3. Precipitation deposited was used to locate active site of immobilized papain. The results show that the macroporous resins carier of immobilized papain leads to greater enzyme activity, and the distribution of activated enzyme is uniform. Most of activated enzyme was immobilized on the macroporous resins. The optimum condition of localization of actitvity of immobilized papain was studied. |
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| Keywords: | X-ray microanalysis Immobilized papain Macroporous resins Enzyme activity |
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