光谱法和色谱法研究酰胺类除草剂与脲酶的结合作用

运用高效液相色谱法和荧光光谱法研究了四种酰胺类除草剂的热力学函数以及与脲酶的作用。研究表明，反相高效液相色谱流动相组成与酰胺类除草剂的容量因子成线性关系，并测定了乙草胺、丙草胺、丁草胺、异丙甲草胺的过量热力学焓变Δ*以及与脲酶的结合常数K和结合位点数n。分析表明过量热力学函数与结合常数K存在较好的相关性。

Study on the Reaction Mechanism of Chloroacetanilide Herbicides with Urease Using Fluorescence Spectrum and High-Performance Liquid Chromatography

The relationship between excess thermodynamic function and binding to urease of four chloroacetanilide herbicides was studied using high-performance liquid chromatography and fluorescence spectrum. The linear relationship between the composition of mobile phase of RP-HPLC and the capacity factor of chloroacetanilide herbicides has been obtained. The excess thermodynamic enthalpy (deltaH* ) of acetochlor, pretilachlor, butachlor and metolachlor was determined, and the binding constant K and the number of binding sites with urease were calculated. The relationship between excess thermodynamic function and the binding constant K was suggested.