吖啶橙共振光散射法测定脱氧核糖核酸

研究了吖啶橙与DNA作用的共振散射光谱，发现在pH 11的碱性溶液中痕量核酸对吖啶橙的共振散射光产生增强作用，且增强程度与核酸浓度之间有良好的线性关系。据此建立了测定核酸的高灵敏共振光增强分析方法。其最大散射波长均位于324 nm处，测定鱼精DNA(fs DNA)的线性范围是3.1×10-8～7.3×10-6 g·mL-1，检测限20.5 ng·mL-1，测定小牛胸腺DNA(ct DNA)的线性范围是7.5×10-8～9.8×10-6 g·mL-1，检测限20.8 ng·mL-1。该方法简单，操作方便，选择性好，灵敏度高，用于合成样品的测定结果满意。

Determination of Deoxyribonucleic Acid with Acridine Orange by Resonance Light Scattering Technique

The resonance light scattering (RLS) spectra of acridine orange with deoxyribonucleic acid have been studied. The RLS of acridine orange is greatly enhanced by deoxyribonucleic acid in pH 11. There is a resonance light scattering peak at 324 nm. Under optimal conditions, the linear ranges of the calibration curves are 7.5 x 10(-8) -9.8 x 10(-6) g x mL(-1) for Calf thymus DNA (ct DNA), 3.1 x 10(-8) -7.3 x 10(-6) g x mL(-1) for Fish sperm DNA (fs DNA) with detection limits 20.8 ng x mL(-1) for ct DNA and 20.5 ng x mL(-1) for fs DNA. This method is simple, selectivity and sensitivity has been applied to the determination of deoxyribonucleic acid in mixed samples with satisfactory results.