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利用吸收光谱确定叶绿素a和b的颜色
引用本文:董金一,程晓舫,符泰然,丁金磊,范学良.利用吸收光谱确定叶绿素a和b的颜色[J].光谱学与光谱分析,2008,28(1):141-144.
作者姓名:董金一  程晓舫  符泰然  丁金磊  范学良
作者单位:1. 中国科学技术大学热科学和能源工程系,安徽,合肥,230027
2. 中国安全生产科学研究院,北京,100029
摘    要:叶绿素a和b在植物生长过程中起着至关重要的作用,精确测定其含量,可为农作物生长状况、植物病理诊断等提供科学依据,是实施精准农业的关键所在。文章将前人已经给出的叶绿素a和叶绿素b的吸收光谱实验数据,转换为透过光谱。用人眼观察其透过光谱,显然就形成叶绿素a和b的颜色。于是,根据色度学的三基色原理,选择具有确定光谱功率分布的标准光源作为照明光源,确定了它们在CIE1931-色品图中的色度坐标:叶绿素a为(0.1981,0.3341),落在蓝绿色区域内;叶绿素b为(0.2704,0.5663),落在黄绿色区域内。上述处理的要点是:将叶绿素a和叶绿素b的光谱曲线压缩成色度图上的坐标点,其结果将为发展植物单叶或群体的非接触式、远距离、无损伤测定植物色素含量的新技术提供必要的理论支持。

关 键 词:颜色  吸收光谱  色度坐标  叶绿素a  叶绿素b
文章编号:1000-0593(2008)01-0141-04
收稿时间:2006-05-10
修稿时间:2006-08-20

Determination of Chlorophyll a and b Using Absorption Spectrum
DONG Jin-yi,CHENG Xiao-fang,FU Tai-ran,DING Jin-lei,FAN Xue-liang.Determination of Chlorophyll a and b Using Absorption Spectrum[J].Spectroscopy and Spectral Analysis,2008,28(1):141-144.
Authors:DONG Jin-yi  CHENG Xiao-fang  FU Tai-ran  DING Jin-lei  FAN Xue-liang
Institution:Department of Thermal Science and Energy Engineering, University of Science and Technology of China, Hefei 230027, China. jydong@mail.ustc.edu.cn
Abstract:Chlorophyll a and chlorophyll b play a significant role in the plant growth process. Precise determination of its content could provide the scientific basis for the corps growth state, the plant pathology diagnosis and so on, and is the key point of implementing accurate agriculture. The authors transfered the absorption spectrum experiment data of chlorophyll a and chlorophyll b determined by predecessor into a transmission spectrum. Observing the transmission spectrum using human eyes, the color of chlorophyll a and chlorophyll b was obtained. Thus, according to the predecessor's experiment curve of absorption spectrum of chlorophyll a and chlorophyll b, combining the three primary color principles of colorimetry, and choosing a standard light source with spectrum power distribution similar to D65 as the illumination, the authors determined their chromaticity coordinates in the 1931CIE-x, y chromaticity diagram: Chlorophyll a is (0.198 1, 0.334 1), which falls in the cyan color region; Chlorophyll b is (0. 270 4, 0. 566 3), which falls in the yellowish green region. The main point of above processing is: reducing the spectrum curves of chlorophyll a and the chlorophyll b to coordinates on the chromaticity diagram, and the result could offer the essential theoretical support for a new non-contact, long-distance and non-damage technology to determine the pigment content in single leaf or mass.
Keywords:Color  Absorption spectra  Chromaticity coordinator  Chlorophyll a  Chlorophyll b
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