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| 马兜铃酸与牛血清白蛋白的相互作用研究 | |
| 引用本文： | 刘家琴,田建袅,边清泉,胡之德.马兜铃酸与牛血清白蛋白的相互作用研究[J].光谱学与光谱分析,2006,26(4):715-719. |
| 作者姓名： | 刘家琴 田建袅 边清泉 胡之德 |
| 作者单位： | 1. 绵阳师范学院化学与化学工程系,四川,绵阳,621000;兰州大学化学化工学院,甘肃,兰州,730000 2. 广西师范大学化学化工学院,广西,桂林,541001 3. 绵阳师范学院化学与化学工程系,四川,绵阳,621000 4. 兰州大学化学化工学院,甘肃,兰州,730000 |
| 基金项目： | 四川省教育厅资助项目 , 绵阳师范学院校科研和教改项目 |
| 摘 要： | 以荧光猝灭方法研究了模拟生理条件下马兜铃酸与牛血清白蛋白(BSA)的相互作用. 研究发现: 马兜铃酸与牛血清白蛋白有强的结合, 结合位置与色氨酸残基间的距离r为2.8 nm;以已知结合位置的标记药物为探针, 确定了马兜铃酸与BSA的结合位置为亚结构域ⅢA;利用CD和FTIR技术考察了马兜铃酸对牛血清白蛋白二级结构的影响, CD结果表明马兜铃酸与BSA的键合使BSA中α-螺旋结构含量从43.5%降到36.7%. |
| 关 键 词： | 马兜铃酸 牛血清白蛋白 相互作用 |
| 文章编号： | 1000-0593（2006）04-0715-05 |
| 收稿时间： | 2005-05-16 |
| 修稿时间： | 2005-08-28 |
Research on the Interaction of Aristolochic Acid and Bovine Serum Albumin |
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| LIU Jia-qin,TIAN Jian-niao,BIAN Qing-quan,HU Zhi-de.Research on the Interaction of Aristolochic Acid and Bovine Serum Albumin[J].Spectroscopy and Spectral Analysis,2006,26(4):715-719. | |
| Authors: | LIU Jia-qin TIAN Jian-niao BIAN Qing-quan HU Zhi-de |
| Institution: | 1. Department of Chemistry, Mianyang Teachers College, Mianyang 621000, China; 2. College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, China ;3. College of Chemistry and Chemical Engineering, Guangxi Normal University, Guilin 541001, China |
| Abstract: | The interaction between aristolochic acid and bovine serum albumin (BSA) under physiological conditions was investigated by fluorescence quenching methods. The results indicate that there is a strong interaction between aristolochic acid and BSA, and the distances between the binding location and tryptophan residue is 2.8 nm. The binding location of aristolochic acid in BSA is in subdomain III A. In addition, the effects of aristolochic acid on the protein second structure were studied using CD and FTIR techniques. The results of CD proved that the alpha-helix contents of BSA decreased from 43.5% to 36.7% after binding with aristolochic acid. |
| Keywords: | Aristolochic acid BSA Interaction |
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