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影响胰岛素荧光光谱的几个物理因素的测定和分析
引用本文:刘义保,陈树德,张红峰,陈家森,常波.影响胰岛素荧光光谱的几个物理因素的测定和分析[J].光谱学与光谱分析,1999,19(3):481-483.
作者姓名:刘义保  陈树德  张红峰  陈家森  常波
作者单位:1. 华东地质学院,344000,临川
2. 华东师范大学物理系,200062,上海
3. 华东师范大学生物系,200062,上海
4. 沈阳体育学院,110032,沈阳
摘    要:胰岛素是调节细胞代谢或生长的重要因子,也是多种细胞体外培养的促生长因子。本文通过荧光光谱,测定和分析了电场、浓度等物理因素对胰岛素荧光光谱的影响。证明电场、浓度和温度都能影响胰岛素荧光光谱,从而影响胰岛素多聚体的构象,并可能影响其功能。

关 键 词:胰岛素  荧光光谱  脉冲电场

Determination and Analysis of Physical Factors Affecting Insulin Fluorescent Spectrum
Yibao LIU,Shude CHEN,Hongfeng ZHANG,Jiasen CHEN,Bo CHANG.Determination and Analysis of Physical Factors Affecting Insulin Fluorescent Spectrum[J].Spectroscopy and Spectral Analysis,1999,19(3):481-483.
Authors:Yibao LIU  Shude CHEN  Hongfeng ZHANG  Jiasen CHEN  Bo CHANG
Institution:East China Geological Institute, 344000 Linchuan.
Abstract:Insulin is an important factor which regulates cell metabolism and growth.The influences on insulin fluorescent spectrum by pulse electrical field,concentration and temperature were determined in this paper.It was proved that pulse electrical field,concentration and temperature could influence insulin fluorescent spectrum,and thus influence insulin confirmation and insulin biological activity.The mechanism of insulin influenced by pulse electrical field is different to temperature.It is possible that the electrical field influences the insulin flnorescent spectrum by Changing tyr position and the temperature influences the insulin flourescent spectrum by molecule thermal motion.The insulin confirmation influenced by electrical fieled is steady.
Keywords:Insulin    Fluorescent spectrum    Pulse electrical field    Concentration    Temperature    Confirmation    Cell activity
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