| 应用FTIR直接测定法鉴定大豆的品种 |
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| 引用本文： | 洪庆红,李丹婷,郝朝运.应用FTIR直接测定法鉴定大豆的品种[J].光谱学与光谱分析,2005,25(8):1246-1249. |
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| 作者姓名： | 洪庆红 李丹婷 郝朝运 |
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| 作者单位： | 1.金华职业技术学院材料与化工学院，浙江 金华 321017
2.浙江师范大学化学与生命科学学院，浙江 金华 321004 |
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| 基金项目： | 浙江省自然科学基金（0304617）资助项目 |
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| 摘 要： | 利用傅里叶变换红外光谱(FTIR)法测定了8个不同大豆品种的子叶及外表皮的红外光谱，对他们的红外光谱进行了分析，并对其吸收峰进行了归属。通过对各谱图的比较分析发现，不同的大豆品种的红外吸收峰形及峰强有所区别。结果表明： 不同的大豆品种的FTIR有明显的差别，特别是1 800～1 200 cm-1范围内有较大的差异，它反映的主要是蛋白质分子的酰胺Ⅰ带及酰胺Ⅱ带附近，这可能是源于基因的差异。因此FTIR法可用于不同大豆品种的鉴别。
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| 关 键 词： | 傅里叶变换红外光谱 直接测定 大豆 品种鉴定 |
| 文章编号： | 1000-0593（2005）08-1246-04 |
| 收稿时间： | 2005-01-17 |
| 修稿时间： | 2005-04-28 |
Identification of Soybean Varieties by Direct Determination of FTIR Spectrum |
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| HONG Qing-hong,LI Dan-ting,HAO Chao-yun.Identification of Soybean Varieties by Direct Determination of FTIR Spectrum[J].Spectroscopy and Spectral Analysis,2005,25(8):1246-1249. |
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| Authors: | HONG Qing-hong LI Dan-ting HAO Chao-yun |
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| Institution: | 1.College of Materials and Chemical Engineering，Jinhua College of Profession and Technology，Jinhua 321017，China2.College of Chemistry and Life Science，Zhejiang Normal University，Jinhua 321004，China |
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| Abstract: | The spectra of cotyledons and outer layer parts of skins of eight kinds of soybeans were determined by FTIR spectrometry with OMNI-sampler directly, fast and accurately. The absorption peaks of the spectra w ere analyzed. Results from the comparison of the spectra showed some differences in the intensity and the shape of absorption peaks of their FTIR spectra, especially in the range of 1800-1200 cm(-1), which mainly reflected the substituent nature of amide I and II of protein. The major differences of spectra in this region might result from the differences in genes. So FTIR spectra could be used for the identification of soybean varieties. |
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| Keywords: | Fourier transform infrared spectroscopy Direct determinaion Soybean Identification of varieties |
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