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| 阿霉素与DNA作用的共振光散射研究及在DNA分析中的应用 | |
| 引用本文： | 冯硕,李正平,张淑红,方正.阿霉素与DNA作用的共振光散射研究及在DNA分析中的应用[J].光谱学与光谱分析,2006,26(4):707-710. |
| 作者姓名： | 冯硕 李正平 张淑红 方正 |
| 作者单位： | 1. 河北大学化学与环境科学学院,河北,保定,071002;河北农业大学理学院生物无机化学实验室,河北,保定,071001 2. 河北大学化学与环境科学学院,河北,保定,071002 3. 河北农业大学理学院生物无机化学实验室,河北,保定,071001 |
| 基金项目： | 中国科学院资助项目 , 河北省自然科学基金 |
| 摘 要： | 研究了抗癌药物阿霉素与DNA相互作用的吸收光谱、荧光光谱和共振光散射光谱, 发现阿霉素与DNA相互作用产生强烈增强的共振光散射信号, 共振光散射技术在研究DNA与阿霉素的相互作用时, 其灵敏度远远高于吸收光谱和荧光光谱. DNA与阿霉素作用在322与564 nm处产生两个共振散射峰, 在弱酸性条件下(pH 5.72), DNA的浓度在0～8.0 μg·mL-1范围内与散射强度呈良好的线性关系, 对小牛胸腺DNA和鱼精子DNA的检出限分别为36.8和40.1 ng·mL-1. 由此建立了一种选择性好, 灵敏度高的DNA共振光散射分析方法. |
| 关 键 词： | 共振光散射 DNA 阿霉素 |
| 文章编号： | 1000-0593（2006）04-0707-04 |
| 收稿时间： | 2005-03-09 |
| 修稿时间： | 2005-06-28 |
Study on Interaction of Adriamycin and DNA with a Resonance Light Scattering Technique and Its DNA Analytical Application |
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| FENG Shuo,LI Zheng-ping,ZHANG Shu-hong,FANG Zheng.Study on Interaction of Adriamycin and DNA with a Resonance Light Scattering Technique and Its DNA Analytical Application[J].Spectroscopy and Spectral Analysis,2006,26(4):707-710. | |
| Authors: | FENG Shuo LI Zheng-ping ZHANG Shu-hong FANG Zheng |
| Institution: | College of Chemistry and Environment Science, Hebei University, Baoding, China. |
| Abstract: | The interaction of adriamycin (ADM) and DNA results in strongly enhanced resonance light scattering (RLS) spectra characterized by two peaks at 322 and 564 nm, respectively. The interaction of ADM and DNA was investigated by using absorption spectra, fluorescence spectra, and RLS spectra. The results indicate that RLS spectra have the highest sensitivity. It was found that the enhanced RLS intensity at 322 nm was proportional to the concentration of DNA in the range of 0-8.0 microg x mL(-1). An RLS method for the determination of DNA was accordingly described. The limit of determination was 36.8 and 40.1 ng x mL(-1) for calf thymus DNA and fish sperm DNA, respectively. The proposed method has a high sensitivity and good selectivity due to the specificity of the interaction of ADM and DNA. |
| Keywords: | Resonance light scattering DNA Adriamycin |
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