氢化物发生-原子荧光光谱法测定椒目中不同化学形态的砷

结合椒目化学成分的分离提取方法，采用AFS-3100原子荧光光谱法，分析了不同产地椒目中的砷含量及其形态。使用0.45 μm滤膜和混合溶剂提取的方法将椒目中的砷分为悬浮态和可溶态，并使用离子交换树脂将可溶态中的砷分为As(Ⅲ)，As(Ⅴ)，MMA，DMA，建立了砷的四种形态分离分析方法。结果显示，各产地椒目中均以无机砷为主且As(Ⅴ), 含量较As(Ⅲ)高，甲醇-水(4∶1)对椒目中砷的浸提率高，椒目水可煎出态总砷百分率为53.7%～62.3%。应用本方法对椒目中的砷进行形态分析，回收率在96.0%～101.3%范围内，RSD小于2.66%。表明其灵敏度高，重现性好。

Determination of Arsenic Species in Bunge Pricklyash Seed by HG-AFS

Some of traditional Chinese medicine (TCM) contain arsenide, such as realgar. The total amount of arsenic in the TCM exceeds the limits according to related regulations. But the roles of arsenic in TCM or its side-effects depend on its species existing in those therapies, not on the total amount of arsenic. Therefore, in recent years, the analysis of arsenic in TCM focuses on the species of arsenic. The present paper combines the method of extracting chemical composition from bunge pricklyash seed and analyzing the contents and the speciation of arsenic of bunge pricklyash seed (BPS) in different habitat by atomic fluorescence spectrometry (AFS-3100). By using 0.45 μm filter membrane and mixed solvent，two different species of the decoction of BPS were extracted，named the suspended and the soluble，and by using ion-exchange resin，the soluble arsenic could be separated as As(Ⅲ), As(Ⅴ), MMA and DMA. The authors established the segregation analysis methods for those species. The results shows that BPS in different habitat all contain arsenic，mostly as the species of inorganic arsenic，and the contents of As(Ⅴ) are higher than As(Ⅲ). The lixiviating rate of arsenic is highest with the solvent of methanol-water(4/1)，reaching 53.7%-62.3%. The recovery of the method was 96.0%-101.3% and the relative standard deviation was less than 2.66%. The results show that the sensitivity and reproducibility of this method were excellent，and provided theoretical basis for further development of BPS.