白僵蚕的红外指纹图谱鉴别研究

采用红外光谱技术研究了健康蚕，不同季节和品种的白僵蚕醇溶部位的红外指纹图谱，采用共有峰率和变异峰率双指标序列法对其图谱的结构特征进行了分析鉴别。结果表明：白僵蚕醇溶部位具有稳定而独特的红外指纹图谱。健康蚕的红外指纹图谱与白僵蚕的红外指纹图谱明显不同，两图谱共有峰率仅为63.0%，变异峰率较大，健康蚕相对于白僵蚕变异峰率达41.2%;不同品种、不同季节的白僵蚕的红外指纹图谱相似，但也存在一定差异，其中不同品种的白僵蚕的红外指纹图谱最低共有峰率为76.0%，最高为92.0%，春季与秋季白僵蚕醇溶部位的红外指纹图谱共有峰率为73.1%。白僵蚕醇溶部位的红外指纹图谱可以用作鉴定白僵蚕，区别不同品种和季节的白僵蚕的一种手段。

Identification of White Muscardin Silkworms by Infrared Spectroscopy

The infrared spectra of the ethanol extracts of well-living silkworms and white muscardin silkworms of different seasons and breeds were analyzed by means of the sequential analysis in which two indexes,i.e.common peak ratio and variant peak ratio,were applied.The results showed that the ethanol extracts of white muscardin silkworm have a stable and distinct infrared spectrum.The spectral differences of the ethanol extracts between white muscardin silkworms and well-living silkworms were so obvious that the common peak ratio of them was no more than 63.0%,and the variant peak ratio amounted to 41.2%.The spectra of different breeds and seasons conformed with each other with a few small differences.The minimum common peak ratio of the spectra of different breeds was 76.0%,and the maximal ratio was 92.0%.The common peak ratio of the spectra of different seasons was 73.1%.Infrared spectrometry was proved to be good for the identification of white muscardin silkworms and the differentiation of white muscardin silkworms of different breeds and seasons.