首页 | 本学科首页   官方微博 | 高级检索  
     检索      

应用圆二色光谱研究电场对辣根过氧化物酶二级结构的影响
引用本文:许强,杨体强,敖敦格日勒,姚占全.应用圆二色光谱研究电场对辣根过氧化物酶二级结构的影响[J].光谱学与光谱分析,2007,27(3):565-568.
作者姓名:许强  杨体强  敖敦格日勒  姚占全
作者单位:1. 内蒙古大学理工学院,内蒙古,呼和浩特,010021
2. 内蒙古农业大学水利与土木建筑工程学院,内蒙古,呼和浩特010018
基金项目:国家自然科学基金 , 内蒙古重大项目
摘    要:辣根过氧化物酶(HRP)经不同强度的电场处理,用圆二色光谱研究了电场对辣根过氧化物酶二级结构的影响.结果表明:在1.0~6.0 kV·cm-1范围,不同强度的电场对辣根过氧化物酶的α-螺旋、β-折叠、β-转角及无规卷曲相对含量的影响程度不同.与对照组相比,处理组的α-螺旋、β-转角和无规卷曲含量降低,降幅范围分别为0.3%~11.4%,1.7%~15.3%和0.9%~20.4%;β-折叠含量增加,增幅范围为0.9%~82.7%.电场作用具有使辣根过氧化物酶的二级结构中α-螺旋、β-转角和无规卷曲向β-折叠转化的趋势.研究结果对解释电场处理种子生物效应具有重要意义.

关 键 词:电场  辣根过氧化物酶  圆二色谱  二级结构
文章编号:1000-0593(2007)03-0565-04
收稿时间:2005-12-06
修稿时间:2006-03-28

Study on the Effect of Electric Field on the Secondary Structure of HRP by Circular Dichroism
XU Qiang,YANG Ti-qiang,AO Dun-ge-ri-le,YAO Zhan-quan.Study on the Effect of Electric Field on the Secondary Structure of HRP by Circular Dichroism[J].Spectroscopy and Spectral Analysis,2007,27(3):565-568.
Authors:XU Qiang  YANG Ti-qiang  AO Dun-ge-ri-le  YAO Zhan-quan
Institution:1. College of Science and Technology, Inner Mongolia University, Huhhot 010021, China 2. College of Water Conservancy and Civil Architecture Engineering, Inner Mongolia Agricultural University, Huhhot 010018, China
Abstract:After the HRP was treated with different strength of electric field, the effect of electric field on the secondary structure of HRP was studied by circular dichroism. The results show that different electric field strength ranging from 1.0 to 6.0 kV x cm(-1) has a different effect on the relative contents of alpha-helix, beta-sheet, beta-turn and random coil of the HRP. As compared with the contrast, the relative contents of alpha-helix, beta-turn and random coil decreased by 0.3%-11.4%, 1.7%-15.3% and 0.9%-20.4% respectively, while beta-sheet content increased by 0.9%-82.7%. The electric treatment tends to transform the alpha-helix, beta-turn and random coil into beta-sheet for the HRP. The result of this study has important meaning to explain the biological effect of electric treatment seeds.
Keywords:Electric field  HRP  CD  Secondary structure
本文献已被 CNKI 维普 万方数据 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号