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维多利亚蓝B与DNA作用的共振光谱特性及分析应用
引用本文:苏界殊,陈小明,龙云飞,李维,罗海棠.维多利亚蓝B与DNA作用的共振光谱特性及分析应用[J].光谱学与光谱分析,2004,24(1):37-40.
作者姓名:苏界殊  陈小明  龙云飞  李维  罗海棠
作者单位:1. 湘潭大学化学学院,湖南,湘潭,411105;湖南科技大学化学系,湖南,湘潭,411201
2. 湘潭大学化学学院,湖南,湘潭,411105
3. 湖南科技大学化学系,湖南,湘潭,411201
基金项目:湖南省自然科学基金 (0 0JJY2 0 84),湖南省教育厅资助项目
摘    要:文章基于维多利亚蓝B与脱氧核糖核酸在弱碱性条件下共振光散射的增强效应。建立了一种测定DNA的共振光散射法。pH值在 9 0~ 10 5范围内 ,三羟甲基氨基甲烷和盐酸 (Tris HCl)缓冲溶液中 ,维多利亚蓝B与DNA分子作用 ,使共振光散射增强 ,存在二个共振光散射增强峰 ,其强度与DNA的浓度呈线性关系 ,线性范围为 0~ 3 0mg·L- 1 ,相关系数为 0 9978,检出限为 2 1 5 μg·L- 1 ,用于fsDNA合成样品的测定获得了满意的结果

关 键 词:维多利亚蓝B  脱氧核糖核酸  共振光散射光谱
文章编号:1000-0593(2004)01-0037-04
修稿时间:2003年7月16日

Resonance Light Scattering Study on the Interaction of Victory Blue-B with Deoxyribonucleic Acid and Application to DNA Assay
SU Jie-shu ,CHEN Xiao-ming ,LONG Yun-fei ,LI Wei ,LUO Hai-tang.Resonance Light Scattering Study on the Interaction of Victory Blue-B with Deoxyribonucleic Acid and Application to DNA Assay[J].Spectroscopy and Spectral Analysis,2004,24(1):37-40.
Authors:SU Jie-shu    CHEN Xiao-ming  LONG Yun-fei  LI Wei  LUO Hai-tang
Institution:Institute of Chemistry, Xiangtan University, Xiangtan 411105, China.
Abstract:The enhancement of the Resonance Light Scattering (RLS) due to Victory blue-B on the molecular surface of DNA in alkalescence solution was studied. A method of resonance light scattering was developed to determine DNA. Deoxyribonucleic acid (DNA) was studied in the range of pH 9.0-10.5. The effects of interference substances and optimization of procedure were investigated and a new method was established for the determination of DNA at nanogram level based on RLS measurement. The linear range of the DNA is 0-3.0 mg x L(-1) with a correlation coefficient of 0.9978. The detection limit is 21.5 microg x L(-1). The method has been applied to the determination of DNA in mixed samples with satisfactory result.
Keywords:Victory blue-B  DNA  Resonance scattering spectroscopy
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