新型冠状病毒S蛋白在金纳米粒子中的表面增强拉曼效应

表面增强拉曼光谱技术因其高灵敏度、操作简单、快速检测等优点，被广泛用于病毒检测方面。国内外的病毒拉曼检测研究主要集中在检测病毒核酸以及组成核酸的各种碱基的表面增强拉曼光谱(SERS)，但少见对病毒蛋白的SERS检测。以新型冠状病毒（SARS-CoV-2）的S蛋白为检测对象，采用无标记SERS检测方法，对比SARS-CoV-2固态、饱和液态S蛋白的普通拉曼光谱和选用40 nm金纳米粒子为基底的SARS-CoV-2低浓度S蛋白SERS光谱。结果表明，以40 nm金纳米粒子为基底，采用SERS技术检测SARS-CoV-2的S蛋白是完全可行的。SARS-CoV-2的S蛋白分子中的羧基与金纳米粒子发生了分子增强，氨基与金纳米粒子发生了电磁增强，从而使得SARS-CoV-2的S蛋白拉曼效应得到了增强，并使得峰位发生一定移动。实验获得了较好的SARS-CoV-2低浓度S蛋白SERS光谱，为建立敏感、特异、快速的SARS-CoV-2检测新技术提供了一种方法。

Surface-enhanced Raman effect of new coronavirus S protein in gold nanoparticles

Surface-enhanced Raman spectroscopy (SERS) technology has been widely used in viral molecular detection due to its high sensitivity, simple operation and rapid detection. The research of virus detection by Raman technology at home and abroad mainly focuses on the detection of the SERS spectrum of viral nucleic acids and various bases that make up the nucleic acids, and detection of viral proteins is rare. In this paper, the S protein of the new coronavirus (SARS-CoV-2) is used as the detection object, and with the label-free SERS detection method, the ordinary Raman spectra of solid and saturated liquid S protein of the SARS-CoV-2 and the SERS spectra of the low-concentration S protein of SARS-CoV-2 on the substrate of gold nanoparticles with a size of 40 nm are compared. The results show that it is completely feasible to use SERS technology to detect the S protein of SARS-CoV-2 on the substrate of 40 nm gold nanoparticles. The carboxyl groups in the S protein molecule of SARS-CoV-2 and gold nanoparticles are molecularly enhanced, and the amino groups and gold nanoparticles are electromagnetically enhanced, so that the Raman effect of the S protein of the SARS-CoV-2 is enhanced and the peak position is moved to a certain extent. The experiments obtained relatively good SERS spectra of the low-concentration S protein of SARS-CoV-2, which provides a method for the establishment of a sensitive, specific and rapid detection technology for the S protein of the SARS-CoV-2.