| Protein–membrane interactions investigated with surface-induced fluorescence attenuation |
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| 引用本文： | 马丽,李颖,李明,胡书新.Protein–membrane interactions investigated with surface-induced fluorescence attenuation[J].中国物理 B,2017,26(12):128708-128708. |
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| 作者姓名： | 马丽 李颖 李明 胡书新 |
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| 作者单位： | 1. Key Laboratory of Soft Matter Physics, Institute of Physics, Chinese Academy of Sciences, Beijing 100190, China;
2. University of Chinese Academy of Sciences, Beijing 100049, China |
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| 基金项目： | Project supported by the National Natural Science Foundation of China (Grant No. 11574382) and the Key Research Program of Frontier Sciences, Chinese Academy of Sciences (Grant No. QYZDJ-SSW-SYS014). |
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| 摘 要： | Research on protein–membrane interactions has been undeveloped due to the lack of proper techniques to detect the position of proteins at membranes because membranes are usually only about 4-nm thick. We have recently developed a new method named surface-induced fluorescence attenuation(SIFA) to track both vertical and lateral kinetics of a single labelling dye in supported lipid bilayers. It takes advantage of strong interaction between a light-emitting dye and a partially reflecting surface. By applying the technique to membrane proteins being fluorescently labelled at different residues, here we show that SIFA can measure not only the insertion depth of a dye inside a lipid bilayer, but also the position of a dye in solution near the surface. SIFA can therefore be used to study membrane proteins of various types.
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| 收稿时间： | 2017-09-10 |
Protein-membrane interactions investigated with surface-induced fluorescence attenuation |
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| Institution: | 1. Key Laboratory of Soft Matter Physics, Institute of Physics, Chinese Academy of Sciences, Beijing 100190, China;
2. University of Chinese Academy of Sciences, Beijing 100049, China |
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| Abstract: | Research on protein-membrane interactions has been undeveloped due to the lack of proper techniques to detect the position of proteins at membranes because membranes are usually only about 4-nm thick. We have recently developed a new method named surface-induced fluorescence attenuation (SIFA) to track both vertical and lateral kinetics of a single labelling dye in supported lipid bilayers. It takes advantage of strong interaction between a light-emitting dye and a partially reflecting surface. By applying the technique to membrane proteins being fluorescently labelled at different residues, here we show that SIFA can measure not only the insertion depth of a dye inside a lipid bilayer, but also the position of a dye in solution near the surface. SIFA can therefore be used to study membrane proteins of various types. |
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| Keywords: | single molecule surfaceinduced fluorescence attenuation membrane-protein interactions molecular mechanism |
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