407 nm 光诱导的红细胞荧光光谱随浓度增加红移的机理分析

用407nm光激发不同浓度离体红细胞可以产生较强的荧光光谱,进一步研究发现这些荧光光谱中存在两个明显的、频谱宽度大致相等的荧光发射区,而且在生理盐水中随着红细胞浓度的增加,其发射的荧光光谱的谱峰值位置向长波方向移动,即出现“红移”现象.从理论上对这种“红移”现象的产生机理进行了分析,表明这种现象是由于浓度变化对荧光团电子能级产生的微扰发生变化所致.该研究结果对现代医学中的荧光光谱诊断技术和低功率激光照射疗法都有一定的参考价值.

Analysis of 407nm Light-induced Erythrocyte Fluorescence Spectra Red Shifted Mechanism with the Concentration Increase

nm light excited erythrocyte fluorescence spectra under different concentration in vitro are presented. It is found that there are two prominent and roughly equal fluorescence-emitting regions within the fluorescence spectra. And the fluorescence spectral peaks are apparently red shifted with the increment of the erythrocyte concentration in physiological brine. Furthermore, physical mechanisms of the spectral peaks red-shifted with the erythrocyte concentrations increasing are analyzed theoretically. Investigation of the fluorescence spectra dependence on the erythrocyte concentrations may makes sense for the light-induced fluorescence spectra for diagnosis and therapy and the low lever laser therapy(LLLT)in vivo or in vitro.