高数值孔径二次谐波显微术的分子取向探测

基于矢量衍射理论和格林函数法,利用坐标变换分析高数值孔径条件下二次谐波强度随胶原纤维取向的变化规律.研究结果表明,当胶原纤维垂直于z轴时,聚焦光场Ey、Ez分量的增强导致二次谐波相对强度极小值的上升；随着胶原纤维极角的改变,二次谐波强度的整体变化趋势发生变化,同时前向和后向二次谐波之间的强度变化也出现明显差异.

Molecule Orientation Determination of Second Harmonic Generation Microscopy Under High Numerical Aperture

Based on the vector diffraction theory and Green function approach,the variation of second-harmonic intensity with the orientation of collagen fiber under high numerical aperture was analysed by use of coordinate transform.The numerical results show that when the orientation of collagen fiber is vertical to the laser propagation direction,the enhancements of the second-harmonic relative intensity minima will increase with the enhancement of Ey and Ex components.For different values of the polar angle of collagen fiber,the variation trends of second-harmonic intensity will change and the difference between the variations of forward and backward second-harmonic intensities occurs.