| 超短光脉冲的平均功率和脉宽及锁模状态对双光子显微镜成象的影响 |
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| 引用本文： | 陈同生,曾绍群,周炜,傅玲,骆清铭.超短光脉冲的平均功率和脉宽及锁模状态对双光子显微镜成象的影响[J].光子学报,2000,29(12):1068-1072. |
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| 作者姓名： | 陈同生 曾绍群 周炜 傅玲 骆清铭 |
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| 作者单位： | [1]生物医学光子教育部重点实验室,武汉 [2]湖北师范学院物理系,黄石 |
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| 基金项目： | 国家自然科学基金!( 39870 2 0 5)，教育部春晖计划资助项目 |
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| 摘 要： | 显微镜成象是研究生物活体细胞结构及活动规律的重要技术.飞秒光脉冲是多光子显微镜的主要光源,它的性能(脉宽、平均功率和锁模状态等)严重影响成象的质量.双光子激发荧光强度正比于超短脉冲平均功率的平方,反比于脉宽,锁模稳定状态严重影响成象的清晰度.对用Cameleon转染的HeLa细胞和GFP转基因水稻叶的实验研究结果与理论一致.
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| 关 键 词： | 超短脉冲 双光子显微镜 |
| 收稿时间： | 2000-01-03 |
| 修稿时间： | 2000-01-03 |
THE EFFECTS OF MEAN POWER,PULSE WIDTH AND MODE LOCKING STATE OF FEMTOSECOND LASER ON TWO PHOTON FLUORESCENCE IMAGING |
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| Chen Tongsheng,Zeng Shaoqun,Zhou Wei,Fu Ling,Luo Qingming.THE EFFECTS OF MEAN POWER,PULSE WIDTH AND MODE LOCKING STATE OF FEMTOSECOND LASER ON TWO PHOTON FLUORESCENCE IMAGING[J].Acta Photonica Sinica,2000,29(12):1068-1072. |
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| Authors: | Chen Tongsheng Zeng Shaoqun Zhou Wei Fu Ling Luo Qingming |
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| Institution: | 1. Institute of Biomedical Photonics, Huazhong University of Science & Technology, Wuhan 430074, China;2. Department of Physics, Hubei Normal University, Huangshi 435002, China |
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| Abstract: | Two photon microscopy is an important tool in studying the structure and function of alive cell.Femtosecond laser is the main excitation optical source in the TPE system.Laser performances,such as mean power,pulse width and mode locking state have strong effects on the quality of fluorescence images.By experiments with standard fluorescence beads and GFP marked rice leaf,it is found that fluorescence intensity of specimen is proportional to the square of the mean power,and inversely proportional to the pulse width of the femtosecond laser.Stable mode locking state improves the contrast of the image. |
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| Keywords: | Ultrashort pulse Two photon microscopyX |
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