三维紫外血浆荧光光谱学特性及机理分析

采用FLS920型多功能光谱仪,得到了220~320 nm紫外光激发的血浆荧光激发与发射的三维光谱图.结果表明,血浆的紫外荧光光谱在334 nm附近存在一个约100 nm宽的谱峰：300~400 nm.该谱峰是由多种荧光团共同作用的结果,血浆的紫外荧光光谱主要是由血浆中高丰度蛋白的色氨酸和酪氨酸的贡献.另外,血浆中存在的其他大量蛋白质和小分子可通过直接发荧光和间接的能量转移或相互作用使血浆荧光光谱谱峰的加宽,即低丰度的、大量蛋白质或小分子的存在,对血浆的荧光光谱特征变化可以起到调制作用.

Characteristics and Mechanism for Three-dimensional UV-fluorescence Spectroscopy of Plasma

The plasma three-dimensional UV fluorescence excitation and emission spectra,the wavelength of 220~320 nm,are obtained by FLS920 Combined Fluorescence Lifetime & Steady State Spectrometer.There is a wide peak with 100 nm in the fluorescence emission spectra near 334 nm,from 300 nm to 400 nm.It is presented that the peak derivers from many fluorophores interaction,which is mostly Tryptophan and tyrosine in plasma protein.In addition,the other proteins and small molecules in the plasma made the fluorescence peak widen by emitting fluorescence and indirect energy transfer or interaction,which may modulate the spectral characteristic.