用荧光探针法研究茶叶花粉黄酮对氧自由基致鼠红细胞膜氧化损伤的保护作用

应用1,6-二苯基-1,3,5-已三烯(DPH)为荧光探针,超氧阴离子自由基和羟基自由基致鼠红细胞膜氧化损伤为模型,研究了茶叶花粉黄酮对鼠红细胞膜氧化损伤的影响.结果表明,超氧阴离子自由基和羟基自由基均能引起鼠红细胞膜脂质过氧化反应,脂质过氧化产物丙二醛含量显著升高,膜脂流动性下降.将鼠红细胞膜预先用茶叶花粉黄酮处理后,膜脂的MDA含量明显下降,呈现剂量与效应关系,膜脂流动性显著提高,表明茶叶花粉黄酮对超氧阴离子自由基和羟基自由基引起的鼠红细胞膜的氧化损伤有保护作用.

THE PROTECTIVE EFFECTS OF TEA POLLEN FLAVONOIDS ON OXIDATIVE INJURY OF ERYTHROCYTE MEMBRANCE (INDUCED BY OXYGEN RADICALS) BY FLUORESCENCE PROBE

diphenyl 1,3,5 hexatriene,a kind of fluorescence probe,was used to observe the effects of tea pollen flavonoids on the oxidative damage in rat erythrocyte ghost induced by peroxy radical and hydroxy radical.The results demonstrated that both peroxy radical and hrdroxy radical increased the content of malondialdehyde(MDA),decreased membrance fluidity significantly,and caused lipid peroxidation of rat erythrocyte ghost.But tea pollen flavonoids could decrease the content of MDA remarkably,improve the membrance fluidity of rat erythrocyte membrance fluidity and inhibite lipid peroxidation in rat erythrocyte membrance.These results suggested that tea pollen was a kind of natural antioxidant and prevent lipid peroxidation in rat erythrocyte membrance damage induced by peroxy radical and hydroxy radical.