83 K光系统Ⅱ核心复合物不同激发的荧光光谱学

在83 K低温下,利用稳态荧光光谱技术对光系统Ⅱ（PSⅡ）核心复合物中激发能的传递进行了研究,激励波长分别选择为436 nm,480 nm,495 nm和507 nm,得到4种波长激发下的稳态荧光光谱.经过比较发现其最大峰值所在的位置没有因激发波长的不同而发生改变,都在696 nm处,在不同激发波长下经过高斯解析获得不同的谱带.根据发射光谱与吸收光谱的对应性,反映了不同的光谱特性,说明在不同波长光的激发下,核心复合物中能量传递的途径不同.同时,可以分析出在核心复合物中,至少有Chl a670.4670,Chl a684.7,685.1683,Chl a689.0687,Chl a690.9,693.4,695.2,698.06904种Chl a组分参与了能量的传递.

Fluorescence Spectroscopy of Photosystem Ⅱ Core Complex with Different Excitation Wavelengths at 83K

At 83 K,fluorescence spectroscopy of the core complex from Photosystem II was studied by the method of steady state fluorescence spectroscopy.The excitation wavelengths were 436 nm,480 nm, 495 nm and 507 nm respectively.The peaks of the four spectra upon different excitation wavelengths are at about 696nm.By Gaussian deconvolution analysis,different spectral bands were analyzed in spectra with different excitation wavelengths,which reflects they have different spectral properties.The way of energy transfer in the core complex of PS II is different when it is excited at different wavelengths.According to the relationship between the emission spectrum and the absorption spectrum, there are at least Chl a670.4670,Chl a684.7,685.1683 ,Chl a689.0687 and Chl a690.9,693.4,695.2,698.0690 involved in the energy transfer in the core complex of PS II .