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应用圆二色光谱研究电场对超氧化物歧化酶二级结构的影响
引用本文:杨体强,许强,敖敦格日勒,姚占全.应用圆二色光谱研究电场对超氧化物歧化酶二级结构的影响[J].光子学报,2007,36(6):1147-1151.
作者姓名:杨体强  许强  敖敦格日勒  姚占全
作者单位:1. 内蒙古大学,理工学院,呼和浩特,010021;内蒙古自治区离子束生物工程重点实验室,呼和浩特,010021
2. 内蒙古大学,理工学院,呼和浩特,010021;宝鸡文理学院,物理系,宝鸡,721007
3. 内蒙古大学,理工学院,呼和浩特,010021
4. 内蒙古农业大学,水利与土木建筑工程学院,呼和浩特,010018
基金项目:国家自然科学基金(30260028),内蒙古大学“513人才”资助
摘    要:超氧化物歧化酶经不同强度的电场作用,采用圆二色光谱研究了不同强度的电场对超氧化物歧化酶二级结构的影响.结果表明:在1.0 kV/cm~6.0 kV/cm范围, 不同强度的电场对超氧化物歧化酶的α-螺旋、β-折叠、β-转角及无规卷曲相对含量的影响程度不同.与对照组相比,处理组的β-折叠和β-转角含量降低,降幅范围分别为2.5%~15.1和0.9%~3.5%;α-螺旋和无规卷曲含量增加,增幅范围分别为3.1%~18.8%和1.0%~8.9%.电场作用具有使超氧化物歧化酶的二级结构中β-折叠、β-转角向α-螺旋及无规卷曲转化的趋势.

关 键 词:电场  超氧化物歧化酶  圆二色谱  二级结构
文章编号:1004-4213(2007)06-1147-5
收稿时间:2005-12-12
修稿时间:2005-12-09

Effect of Electric Field on the Secondary Structure of SOD by Circular Dichroism
YANG Ti-qiang,XU Qiang,GE Ri-le,YAO Zhan-quan.Effect of Electric Field on the Secondary Structure of SOD by Circular Dichroism[J].Acta Photonica Sinica,2007,36(6):1147-1151.
Authors:YANG Ti-qiang  XU Qiang  GE Ri-le  YAO Zhan-quan
Institution:1.College of Science and Technology , Inner Mongolia University, Huhhot 010021 ,China;2. Key Laboratory of Ion Beam Bioengineering Inner Mongolia Autonomous Region, Inner Mongolia University, Hohhot 010021 ,China;3. Physics Department, Baoji College of Art and Science, Baoji, Shaanaci 721007, China;4 .College of Water Conservancy and Civil Architecture Engineering, Inner Mongolia Agricultural University,Huhhot 010018 ,China
Abstract:After the SOD was treated with different strength of electric field,the effect of electric field on the secondary structure of SOD was studied through Circular Dichroism.The results show that different electric field strengths with ranges from 1.0 kV/cm to 6.0 kV/cm have different effects on the relative contents of α-helix,β- sheet,β-turn and random coil of the SOD.As compared with the contrast,the relative contents of β-sheet and β-turn have been decreased to 2.5%~15.1% and 0.9%~3.5%,respectively,and the α-helix and random coil content were increased by 3.1%~18.8% and 1.0%~8.9%,respectively.The electric treatment tends to transform theβ-sheet,β-turn intoα-helix and random coil for the SOD.
Keywords:Electric field  SOD  CD  Secondary structure
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