| 氦氖激光对链霉菌原生质体种间融合频率的影响 |
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| 引用本文： | 陈五岭,姚胜利.氦氖激光对链霉菌原生质体种间融合频率的影响[J].光子学报,1998,27(5):412-417. |
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| 作者姓名： | 陈五岭 姚胜利 |
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| 作者单位： | 1. 西北大学生物系 西安 710069;2. 中国科学院西安光学精密机械研究所 西安 710068 |
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| 摘 要： | 采用双亲灭活原生质体融合技术对金霉素链霉菌(Streptomyces aureofaciens)和龟裂链霉菌(Streptomyces rimous)种间原生质体融合进行了研究,分别利用聚乙二醇(PEG)、He-Ne激光以及PEG与He Ne激光混合诱导融合,结果表明:PEG及激光的双重诱导作用,使链霉菌原生质体种间融合频率得到显着提高。通过同工酶谱分析对融合子进行了初步鉴定。
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| 关 键 词： | He-Ne激光 原生质体 融合频率 |
| 收稿时间： | 1997-11-04 |
EFFECTS OF He-Ne LASER ON FREQUENCY OF INTERSPECIFIC PROTOPLAST FUSIONS OF STREPTOMYCES |
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| Chen Wuling,Luo Wen,Yao Shengli.EFFECTS OF He-Ne LASER ON FREQUENCY OF INTERSPECIFIC PROTOPLAST FUSIONS OF STREPTOMYCES[J].Acta Photonica Sinica,1998,27(5):412-417. |
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| Authors: | Chen Wuling Luo Wen Yao Shengli |
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| Institution: | 1. Department of Biology, Northwest University, Xi’an 710069;2. Xi’an Institute of Optics &; Precision Mechanics, Academia Sinica, Xi’an 710068 |
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| Abstract: | In this paper,interspecific fusions of inactivated protoplasts of both parents streptomyces aureofaciens and S.rimous were studied.Fusions were induced by PEG,He-Ne laser separately or PEG and He-Ne laser together,the results showed that the frequency of interspecific protoplast fusion of Streptomyces was obviously increased under the twofold induction of PEG and He-Ne laser.The fusants were identified by analysing isozyme patterns. |
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| Keywords: | He-Ne laser Protoplast Fusion frequency |
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