He-Ne激光和紫外线对PHB产生菌的复合诱变效应

采用紫外线、He-Ne激光及紫外线与He-Ne激光复合作用的方法, 对本实验室的一株产PHB的细菌G-Q7进行诱变, 以提高其产量. 结果表明, 紫外线和He-Ne激光单独作用, 诱变效果不佳, 而二者的复合作用却能产生很好的效果. 用He-Ne激光辐照20 min再经紫外线照射150 s, 正变菌株的细胞量为11.5g/L, PHB含量为65%, 并具有良好的遗传稳定性. 对诱变前后菌株积累的PHB产物的外观、热性质和13C-NMR图谱进行分析, 结果表明该诱变使代谢途径中的某种酶发生了改变.

The Mutagentc Effect on a Strain Producing PHB by Cooperation of UV and He-Ne Laser

The strain G-Q7 producing PHB was irradiated respectively by UV, He-Ne laser and both of them to increase PHB production. The results indicated that the coordinative effect of UV and He-Ne laser was better than using them individually. The cells were treated firstly by He-Ne laser for 20 minutes and then by UV for 150 seconds, which resulted in a final cell concentration of 11.5 g/L, PHB content of 65%. The mutant had better hereditary stability. The two PHB polymers obtained from the strains G-Q7 and the mutant were analysed by the method of DSC and NMR, the conclusion appears that the mutagenic effect makes some certain enzyme of metabolism change.