| 单细胞凝胶电泳其及光学图像分析 |
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| 引用本文： | 张炜,代正民,罗明智,齐浩.单细胞凝胶电泳其及光学图像分析[J].光子学报,2004,33(7):877-879. |
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| 作者姓名： | 张炜 代正民 罗明智 齐浩 |
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| 作者单位： | 西北大学光子学与光子技术研究所,西安,710069;陕西师范大学生命科学学院,西安,710062;西北大学光子学与光子技术研究所,西安,710069;陕西师范大学生命科学学院,西安,710062 |
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| 基金项目： | SupportedbytheNationalNaturalScienceFoundationofChina(code:30 2 70 383)andimportantitemofShaanxiNormalUniversity |
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| 摘 要： | 采用单细胞凝胶电泳检测DNA损伤度,运用光学图像处理软件对彗星试验得到的参数进行图像分析,结果表明:该方法快捷,灵敏度高,能够较好地应用于DNA损伤度的修复、细胞遗传、环境检测等方面.
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| 关 键 词： | 彗星试验 单细胞凝胶 图像分析 |
| 收稿时间： | 2004-03-10 |
A Single Cell Gel Electrophoresis of Its Optical Image Analysis |
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| Abstract.A Single Cell Gel Electrophoresis of Its Optical Image Analysis[J].Acta Photonica Sinica,2004,33(7):877-879. |
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| Authors: | Abstract |
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| Institution: | (1 Institute of Photonics &; Photon-Technology,Northwest University,Xi′an 710069)
(2 College of Life Science, Shannxi Normal University,Xi′an 710062) |
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| Abstract: | A single cell gel electrophoresis was used to measure DNA damage in individual cells. The extent of DNA damage was quantified by the various parameter which can be done with computerized image analysis system. It was shown that:as a rapid, sensitive and simple tool that can be utilized to nucleus DNA damage test, cell genetics, environmental monitoring. |
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| Keywords: | Comet assay Single cell gel electrophoresis Image analysis |
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