| 紫外激光等选育生淀粉糖化酶高产菌株及其应用 |
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| 引用本文： | 郭爱莲,郭廷巍.紫外激光等选育生淀粉糖化酶高产菌株及其应用[J].光子学报,1999,28(9):780-784. |
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| 作者姓名： | 郭爱莲 郭廷巍 |
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| 作者单位： | 西北大学生物学系!西安710069 |
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| 摘 要： | 以紫外线、LiCl、5氟尿嘧啶、HNO2为诱变剂,黑曲霉Sx为出发菌株,选育出生淀粉糖化酶酶活力提高12%的突变株黑曲霉S’x制取黑曲霉S’x的原生质体,用HeNe激光照射,经反复筛选得到突变株黑曲霉Sy,其酶活力较出发菌株提高51%,而酸性蛋白酶活力降低45%并对其在农作物秸杆的应用进行了研究.
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| 关 键 词： | 紫外 He-Ne激光 HNO2 黑曲霉应用 |
| 收稿时间： | 1999-06-02 |
| 修稿时间： | 1999-06-02 |
SCREENING OF HIGH RAW STARCH GLUCOAMYLASE PRODUCING STRAIN BY UV AND He Ne LASER IRRADIATION AND ITS APPLICATION |
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| Guo Ailian,Guo Tingwei,Sun Xianfeng,Yang Lin,He Jun.SCREENING OF HIGH RAW STARCH GLUCOAMYLASE PRODUCING STRAIN BY UV AND He Ne LASER IRRADIATION AND ITS APPLICATION[J].Acta Photonica Sinica,1999,28(9):780-784. |
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| Authors: | Guo Ailian Guo Tingwei Sun Xianfeng Yang Lin He Jun |
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| Institution: | Department of Biology, Northwest University, Xi’an 710069 |
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| Abstract: | A mutant strain A. niger Sx’ with higher yield raw-starch glucoamylase was obtained from wild strain of A. niger Sx by UV,LiCl,5-Fu and HNO2 treatment Its enzyme activity increased by 12% After many times of He-Ne Laser irradiation to protoplast of A. niger Sx’,A. niger Sy was obtained Its raw-starch glucoamylase activity increased 51% than that of the original strain A. niger Sx,whereas A. niger Sy showed reductions in acid-protease productivity of 45% Furthermore,applications of A. niger Sy on the treatment of the grain stalks studied. |
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| Keywords: | UV lrridiation He Ne laser HNO 2 A niger Application |
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