| 基于相移技术的显微数字全息重构细胞相位 |
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| 引用本文： | 钱晓凡,张磊,董可平.基于相移技术的显微数字全息重构细胞相位[J].光子学报,2006,35(10):1565-1568. |
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| 作者姓名： | 钱晓凡 张磊 董可平 |
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| 作者单位： | 昆明理工大学理学院,昆明,650051 |
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| 基金项目： | 云南省教育厅科研基金（03Y225A）资助 |
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| 摘 要： | 介绍了用显微镜物镜、压电陶瓷和CCD建立的一套测量细胞相位的显微数字全息光路，基于相移技术，给出了重构相位的理论分析，并用洋葱磷片叶细胞作为测试样品，完成了测量细胞相位的实验.结果表明：该系统可以完成细胞相位重构，系统分辨率不低于1 μm.
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| 关 键 词： | 显微数字全息 相位重构 细胞 相移技术 |
| 收稿时间： | 2006-02-21 |
| 修稿时间： | 2006年2月21日 |
Phase Reconstruction of Cells with Microscopy Digital Holography Based on Phase Shifting Technique |
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| Qian Xiaofan,Zhang Lei,Dong Keping.Phase Reconstruction of Cells with Microscopy Digital Holography Based on Phase Shifting Technique[J].Acta Photonica Sinica,2006,35(10):1565-1568. |
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| Authors: | Qian Xiaofan Zhang Lei Dong Keping |
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| Institution: | The Faculty of science, Kunming University of Science and Technology, Kunming 650051 |
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| Abstract: | An optical device designed to measure the phases of cells was introduced,which was made up of objective,PZT,CCD and computer. The phase reconstruction method and related analysis were presented,which was based on the phase shifting technique. The experiment of phase reconstruction of cells using this optical system was done. The results prove that the resolution of this system is higher then 1 μm and reconstructed phase of cells is well. |
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| Keywords: | Microscopy digital holography Phase reconstruction Cells Phase shifting technique |
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