小型化望远式激光共焦扫描显微内窥镜

研制了一种激光共焦扫描显微内窥镜,采用望远式显微内窥光学系统,同时实现长距离的图像中继传输、远心f-theta光学扫描和显微内窥成像功能.二维共焦扫描由双振镜实现,低噪音扫描控制信号由嵌入式系统产生.为实现便携式应用,激光共焦扫描显微内窥镜采用小型化设计方案.首先,体内的显微内窥成像光学系统,外径尺寸为8 mm,工作长度为250.3 mm,可通过标准腹腔镜手术孔进行体内显微内窥成像;其次,采用3 mm通光孔径的小尺寸平面反射镜实现体外共焦扫描,摆动频率为100 Hz,实现快速共焦扫描;最后,激光控制和荧光探测仅通过电缆和光纤与共焦扫描显微内窥镜前端连接,减小了显微内窥镜的前端尺寸和重量.通过实验验证,本系统的成像视场为φ 600 μm,光学分辨率为2.2 μm,可采用手持式或者其他方式工作,进行体内组织的共焦扫描成像,实现微创、在体的荧光显微内窥术.

Miniature Telecentric Confocal Laser Scanning Endomicroscopy

A confocal laser scanning endomicroscopy is developed, which employs a telecentric endomicroscopic optical system to achieve long-distance relay transmission of images, telecentric f-theta optical scanning and endomicroscopic imaging functions. Two-dimensional confocal scanning is realized through two scanning mirrors, and low-noise control signals are generated by the embedded system. Miniaturized design is adopted in the confocal laser scanning endomicroscopy in order to easily use for portable applications. Firstly, the outer diameter of the optical system is 8 mm, and working length of which is 250.3 mm, so in-vivo endomicroscopic imaging can be realized through standard laparoscopic surgery port. Secondly, two small mirrors with aperture of 3mm are employed to achieve in vitro confocal scanning, with the maximum frequency of 100 Hz. Finally, laser system and fluorescence detection are connected with the distal end of the confocal laser scanning endomicroscopy through an optical fiber and a cable, and the size and the weight of the distal end are minimized by this method. The experimental results show that the system's filed of view reaches φ600 μm, and its optical resolution is 2.2 μm. This system can be used under handheld operation or other ways to realize minimally invasive in-vivo fluorescence endoscopic surgery since its miniature size and low weight.