He-Ne激光对增强UV-B辐射拟南芥抗氧化系统的损伤修复

以培养6周左右的拟南芥为材料,采用UV-B辐射（剂量1 KJ/m2/d）和He-Ne激光器（波长632.8 nm,输出功率5 mW·mm2,辐照时间60 s）对材料进行处理,分成CK（没有经过UV-B或激光辐照）组、B(UV-B辐射)组、BL（UV-B和激光复合处理）组和L（激光辐照）组4个不同处理组.结果表明:增强的UV-B辐射拟南芥幼苗导致MDA(Malondialdehyde)、超氧阴离子含量升高,GSH(Glutathione)含量降低,PAL(phenylalanine ammomia-lyase)、CAT(catalase)和APX(ascorbate peroxidase)活性升高,SOD(supemxide dismutas)活性降低.单独He-Ne激光处理使MDA、超氧阴离子含量降低,GSH的含量升高,SOD、APX、CAT的活性升高,PAL的活性降低.UV-B辐射后再用He-Ne激光进行后处理,发现与单独UV-B辐照处理相比,MDA、超氧阴离子含量降低,GSH含量升高,SOD、APX、CAT的活性升高了,PAL的活性降低了.因此激光在一定程度上提高了拟南芥叶片抗氧化能力,在此基础上讨论了其可能的形成机理.

Ramage Repair for Enhanced Ultraviolet-B Radiation on Antioxidant System of Arabiodopsis by He-Ne Laser

Taking Arabidopsis trained about six weeks as the material,UV-B radiation(1 kJ/m2/d) and He-Ne laser irradiation(wavelength 632.8 nm output power 5 mW·mm2,irradiation time 60 s) were used to process the material.The Arabidopsis were divided into four treatment groups including CK(not treated ),L(He-Ne laser),B(UV-B),and BL(UV-B +He-Ne laser).The experimental results show that enhanced UV-B increases the contents of MDA,O.－2and the activities of PAL,CAT,APX,while SOD activity and the content of GSH decrease.But He-Ne laser irradiation reduces the contents of MDA and O.－2,the PAL activity,and increases SOD activity,APX activity,CAT activity,the content of GSH.In contrast to B group,both enhanced UV-B and He-Ne laser have similar results which indicated by He-Ne laser irradiation.The results prove that laser can improve the ability of antioxidant system in Arabidopsis.