Effect of storage conditions of skin samples on their optical characteristics |
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Authors: | E A Genina A N Bashkatov V I Kochubey and V V Tuchin |
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Institution: | 1.Chernyshevsky State University,Saratov,Russia |
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Abstract: | The optical characteristics of skin samples are experimentally studied ex vivo and in vitro at different storage conditions. The experiments are performed on a Cary-2415 spectrophotometer in the spectral range 400–700
nm. Based on the measured diffuse reflectance and total transmittance spectra, the spectra of the absorption and reduced scattering
coefficients are calculated in terms of the inverse adding-doubling method. It is shown that the method of storage of samples
mainly affects the reduced scattering coefficient of biotissue. Thus, upon storage of skin in an isotonic solution and in
its absence, the reduced scattering coefficient increases ∼1.5 and ∼2 times, respectively, compared to the value of this parameter
for the intact sample. The differences in the absorption spectra of ex vivo samples and samples stored under different conditions are the most noticeable in the absorption range of blood and are significant
above 600 nm. |
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