超氧化物歧化酶在L-半胱氨酸修饰金电极上电化学行为的现场拉曼光谱研究

在以电化学方法确认L半胱氨酸对铜锌超氧化物歧化酶(copper,zincsuperoxidedismutase,SOD)在金丝电极上的电子迁移过程起促进作用的基础上,本文论述了将拉曼光谱电化学方法应用于猪红细胞SOD在经L半胱氨酸修饰的金丝电极上的电化学行为的现场拉曼光谱研究。研究结果从分子水平上提供了有关L半胱氨酸修饰在金电极上的电化学过程中,以及SOD在L半胱氨酸修饰的金电极上发生电子迁移过程中,存在于SOD、L半胱氨酸与金电极之间的相互成键作用情况随电化学过程进行而发生变化的微观结构信息,并进行了必要的分析和探讨。

In-situ Raman Spectroscopic Study on the Electrochemical Behaviors of SOD on the L-Cysteine Modified Gold Electrode

As the basis of our present work, it was found that L cysteine can efficiently promote the electron transfer reaction of copper, zinc superoxide dismutase (SOD) on a gold wire electrode. In this paper, to reveal the microscopic mechanism of this promotion, electrochemical in situ Raman spectroscopic technique has been constructed and applied to study the bioelectrochemical processes of SOD. The Raman spectroscopic results, which are related to following two processes: (1) cylic voltammetric measurements of L cysteine when it is modified on a bare gold electrode, (2) cylic voltammetric measurements of SOD in the presence of L cysteine modified gold electrode, are presented. As a result, some possible interactions among SOD, L cysteine and a gold electrode during these cylic voltammetric processes are suggested, and some necessary discussion has been made to elucidate these obtained results.