基于SERS光谱的银溶胶溶液中腺嘌呤的定量分析

碱基是构成核酸的物质基础。碱基的含量分析对于生物体的生理活动及新陈代谢过程研究具有重要意义。本文利用表面增强拉曼散射(SERS)光谱技术研究了腺嘌呤在相对稳定的银溶胶溶液中的SERS光谱信号强度与浓度的定量关系。研究表明添加聚合物作为稳定剂的银溶胶具有良好的稳定性。进一步研究表明, 在1×10-4~ 1×10-3mol ·L-1的浓度范围内, 以吡啶作为内标, 分析物腺嘌呤与内标物特征谱峰强度之比与腺嘌呤的浓度之间呈现良好的线性关系。

Quantitative Analysis with Adenine in Silver Colloidal Sol by Surface-enhanced Raman Scattering Spectroscopic Technique

Surface-enhanced Raman scattering(SERS) spectroscopic technique was used to perform quantitative analyses with adenine,an important basic group from nucleic acid,by using silver colloidal sol as the SERS substrate.Sodium polyacrylate was added into the silver colloidal sol in order to improve its stability.In addition,during different SERS measurements,pyridine was used an internal standard to eliminate the impact of the variations in laser power,optical alignment and focusing on the SERS signal intensity of the analyte.It reveals that a good linear relationship is presented between the concentration of adenine and the characteristic SERS signal intensity ratio of the analyte adenine(733 cm-1) to the internal standard pyridine(1003 cm-1) in the range of 1×10-4~ 1×10-3mol ·L-1.