| 光谱法研究橙皮素与DNA分子间的相互作用机制 |
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| 引用本文： | 刘娟,林琳,王利丹,汤超.光谱法研究橙皮素与DNA分子间的相互作用机制[J].光散射学报,2011,23(1):90-94. |
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| 作者姓名： | 刘娟 林琳 王利丹 汤超 |
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| 作者单位： | 郑州大学化学系,郑州,450052 |
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| 摘 要： | 以盐酸小檗碱(BR)为荧光探针,研究了鲱鱼精DNA与橙皮素的相互作用.采用荧光光谱,紫外可见吸收光谱,盐效应,Scatchard方程等手段,探讨了橙皮素与DNA的作用机制.结果表明,在生理酸度(pH=7.4)下,橙皮素对DNA-BR体系的荧光有猝灭作用,猝灭类型为静态猝灭与动态猝灭共存的模式,其作用方式为嵌插和静电作用...
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| 关 键 词： | 橙皮素 鲱鱼精DNA 荧光光谱 盐酸小檗碱 |
| 收稿时间： | 2010/1/18 |
Spectroscopic Studies on the Mechanism of Interaction Between Hesperidin and DNA |
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| LIU Juan,LIN Lin,WANG Li-dan,TANG Chao.Spectroscopic Studies on the Mechanism of Interaction Between Hesperidin and DNA[J].Chinese Journal of Light Scattering,2011,23(1):90-94. |
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| Authors: | LIU Juan LIN Lin WANG Li-dan TANG Chao |
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| Institution: | (Department of Chemistry,Zhengzhou University,Zhengzhou 450052,China) |
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| Abstract: | The interaction of Hesperidin with herring sperm DNA has been studied by fluorescence method using berberine(BR) as a fiuorescence probe.The mechanism of the interaction between Hesperidin and DNA was also researched by fluorescence spectrum,absorption spectrum,Scatchard equation,and salt effect.The results indicated that under the condition of physiological acidity(pH=7.4),the fluorescence quenching model between HSP and DNA-BR belongs to a mixture of static fluorescence quenching and dynamic fluorescence quenching,HSP interacts with DNA by intercalation and electrostatic gravity.The binding constant calculated was 1.83×104 L ·mol-1. |
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| Keywords: | hesperidin herring sperm DNA fluorescence spectrums berberine hydrochloride |
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