Accurate Measurement of Small Spin–Spin Couplings in Partially Aligned Molecules Using a Novel J-Mismatch Compensated Spin-State-Selection Filter |
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Authors: | Bernhard Brutscher |
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Institution: | Institut de Biologie Structurale, Jean-Pierre Ebel C.N.R.S.—C.E.A. 41, rue Jules Horowitz, Grenoble Cedex, 38027, France |
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Abstract: | The accurate measurement of small spin–spin coupling constants in macromolecules dissolved in a liquid crystalline phase is important in the context of molecular structure investigation by modern liquid state NMR. A new spin-state-selection filter, DIPSAP, is presented with significantly reduced sensitivity to J-mismatch of the filter delays compared to previously proposed pulse sequences. DIPSAP presents an attractive new approach for the accurate measurement of small spin–spin coupling constants in molecules dissolved in anisotropic solution. Application to the measurement of 15N–13C′ and 1HN–13C′ coupling constants in the peptide planes of 13C, 15N labeled proteins demonstrates the high accuracy obtained by a DIPSAP-based experiment. |
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Keywords: | Abbreviations: liquid state NMRAbbreviations: spin-state selectionAbbreviations: molecular alignment residual dipolar couplingAbbreviations: TROSY |
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