Gradient-Enhanced One-Dimensional Proton Chemical-Shift Correlation with Full Sensitivity

High-quality spectra were obtained by implementing pulsed field gradients (PFGs) as part of 1D selective experiments. The use of PFGs for coherence rejection rather than coherence selection ensures that there is no loss of signal and the sensitivity of these experiments is the same as that of their phase-cycled predecessors. The excitation scheme chosen ensures that these experiments are highly resistant to spin–spin relaxation. The following techniques are described: 1D ge-TOCSY, 1D ge-NOESY, 1D ge-TOCSY–TOCSY, 1D ge-NOESY–NOESY, 1D ge-TOCSY–NOESY, and 1D ge-NOESY–TOCSY. Their applications, for the separation of overlapping spin systems, tracing spin-diffusion signals, and extending the transfer of magnetization beyond an individual spin system, are illustrated using oligo- and polysaccharide samples.