Three-dimensional weight-accumulation algorithm for generating multiple excitation spots in fast optical stimulation |
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Authors: | Yu Takiguchi Haruyoshi Toyoda |
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Institution: | 1.Central Research Laboratory,Hamamatsu Photonics K.K.,Hamamatsu,Japan |
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Abstract: | We report here an algorithm for calculating a hologram to be employed in a high-access speed microscope for observing sensory-driven synaptic activity across all inputs to single living neurons in an intact cerebral cortex. The system is based on holographic multi-beam generation using a two-dimensional phase-only spatial light modulator to excite multiple locations in three dimensions with a single hologram. The hologram was calculated with a three-dimensional weighted iterative Fourier transform method using the Ewald sphere restriction to increase the calculation speed. Our algorithm achieved good uniformity of three dimensionally generated excitation spots; the standard deviation of the spot intensities was reduced by a factor of two compared with a conventional algorithm. |
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