In situ observation of photo-bleaching in human single living cell excited by a NIR femtosecond laser |
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Authors: | Sung-Hak Cho Won-Seok Chang Kyoung-Hyun Whang Seong-Hyang Sohn |
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Institution: | a Nano Machining Laboratory, KIMM (Korea Institute of Machinery and Material), 171 Jang-dong, Yuseong-Gu, Daejeon 305-343, Republic of Korea b Laboratory of Cell Biology, Institute for Medical Sciences, Ajou University School of Medicine, Woncheon-dong, Youngtong-gu, San 5, Suwon 443-721, Republic of Korea |
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Abstract: | The photo-bleaching of single living cells excited by femtosecond laser irradiation was observed in situ to study the nonlinear interaction between ultrafast laser pulses and living human breast MDA-MB-231 cells. We conducted a systematic study of the energy dependence of plasma-mediated photo-disruption of fluorescently labeled subcellular structures in the nucleus of living cells using near-infrared (NIR) femtosecond laser pulses through a numerical aperture objective lens (0.75 NA). The behavior of photo-bleached living cells with fluorescently labeled nuclei was observed for 18 h after femtosecond laser irradiation under a fluorescence microscope. The photo-bleaching of single living cells without cell disruption occurred at between 470 and 630 nJ. To study the photo-disruption of subcellular organelles in single living cells using the nonlinear absorption excited by a NIR femtosecond laser pulse, the process of photo-bleaching without photo-disruption provides key information for clarifying the nonlinear interaction between NIR ultrashort, high-intensity laser light and transparent fluorescently labeled living cells. |
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Keywords: | 87 17 &minus d 52 38 Mf 42 62 Be |
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