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Surface modification of PDMS using atmospheric glow discharge polymerization of tetrafluoroethane for immobilization of biomolecules |
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| Authors: | V AnandS Ghosh M Ghosh GM Rao R RailkarRR Dighe |
| Institution: | a Plasma Process Laboratory, Department of Instrumentation and Applied Physics, Indian Institute of Science, Bangalore, 560012, India b Multidisciplinary and Multiscale Device and Design Laboratory (M2D2), Department of Mechanical Engineering, Indian Institute of Science, Bangalore, 560012, India c Structure Function Analysis Lab, Department of Molecular Reproduction Development and Genetics, Indian Institute of Science, Bangalore, 560012, India |
| Abstract: | In this study an atmospheric glow discharge with a fluorocarbon gas as precursor was used to modify the surface of polydimethyl siloxane (PDMS -(CH3)2SiO]n-). The variation in protein immobilizing capability of PDMS was studied for different times of exposure. It was observed that the concentration of proteins adsorbed on the surface varied in an irregular manner with treatment time. The fluorination results in the formation of a thin film of fluorocarbon on the PDMS surface. The AFM and XPS data suggest that the film cracks due to stress and regains its uniformity thereafter. This Stranski-Krastanov growth model of the film was due to the high growth rate offered by atmospheric glow discharge. |
| Keywords: | APGD PDMS Protein Surface modification Wettability |
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